Development of a multiple immunoaffinity column for simultaneous determination of multiple mycotoxins in feeds using UPLC-MS/MS

文献类型： 外文期刊

第一作者： Hu, Xiaofeng

作者： Hu, Xiaofeng;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Hu, Xiaofeng;Zhang, Zhaowei;Li, Peiwu;Hu, Xiaofeng;Zhang, Zhaowei;Li, Peiwu;Zhang, Qi;Hu, Xiaofeng;Li, Peiwu;Hu, Rui;Wang, Min

作者机构：

关键词： Multiple mycotoxins;Feed;Simultaneous determination;Multiple immunoaffinity columns;UPLC-MS/MS

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A sensitive and specific immunoaffinity column to clean up and isolate multiple mycotoxins was developed along with a rapid one-step sample preparation procedure for ultra-performance liquid chromatography-tandem mass spectrometry analysis. Monoclonal antibodies against aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, zearalenone, ochratoxin A, sterigmatocystin, and T-2 toxin were coupled to microbeads for mycotoxin purification. We optimized a homogenization and extraction procedure as well as column loading and elution conditions to maximize recoveries from complex feed matrices. This method allowed rapid, simple, and simultaneous determination of mycotoxins in feeds with a single chromatographic run. Detection limits for these toxins ranged from 0.006 to 0.12 ng mL(-1), and quantitation limits ranged from 0.06 to 0.75 ng mL(-1). Concentration curves were linear from 0.12 to 40 mu g kg(-1) with correlation coefficients of R-2>0.99. Intra-assay and inter-assay comparisons indicated excellent repeatability and reproducibility of the multiple immunoaffinity columns. As a proof of principle, 80 feed samples were tested and several contained multiple mycotoxins. This method is sensitive, rapid, and durable enough for multiple mycotoxin determinations that fulfill European Union and Chinese testing criteria.

分类号： O65

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