Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp citrulli

文献类型： 外文期刊

第一作者： Zeng, Haijuan

作者： Zeng, Haijuan;Li, Jianwu;Zhai, Xuzhao;Song, Chunmei;Liu, Qing;Guo, Wenbo;Liang, Beibei;Zhao, Wenjun;Fan, Enguo

作者机构：

关键词： Acidovorax avenae subsp;citrulli;Monoclonal antibody;Self-paired;Colloidal gold;Immunoassay;Test strip

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We screened a highly specific monoclonal antibody (McAb), named 6D, against Acidovorax avenae subsp. citrulli (Aac). Single McAb 6D was used as both nanogold-labeled antibody and test antibody to develop a single self-paired colloidal gold immunochromatographic test strip (Sa-GICS). The detection limit achieved using the Sa-GICS approach was 10(5) CFU/mL, with a result that can be observed by the naked eye within 10 min. Moreover, Sa-GICS can detect eight strains of Aac and display no cross-reactions with other pathogenic plant microorganisms. Artificial contamination experiments demonstrated that Sa-GICS would not be affected by impurities in the leaves or stems of the plants and were consistent with the PCR results. This is the first report on the development of a colloidal gold immunoassay strip with self-paired single McAb for the rapid detection of Aac.

分类号： O65

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