DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis
文献类型: 外文期刊
第一作者: Qiu, Lingling
作者: Qiu, Lingling;Xu, Lu;Chang, Guobin;Gu, Qixin;Bi, Yulin;Zhang, Yu;Lu, Wei;Ren, Lichen;Zhu, Pengfei;Mu, Yun;Zhang, Yang;Xu, Qi;Chen, Guohong;Liu, Xiangping;Wang, Hongzhi;Wang, Kehua
作者机构:
关键词: Chicken;DNA methylation;Piwil1;Spermatogenesis
期刊名称:JOURNAL OF REPRODUCTION AND DEVELOPMENT ( 影响因子:2.214; 五年影响因子:2.221 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 promoter in five different spelmatogenic cell lines using direct bisulfite pyrosequencing and determined that methylation correlates negatively with germ cell type-specific expression patterns of piwil1. We demonstrated that methylation of the -148 CpG site, which is the predicted binding site for the transcription factors TCF3 and NRF1, was differentially methylated in different spermatogenic cells. This site was completely methylated in PGCs (primordial germ cells), but was unmethylated in round spermatids. A similar result was obtained in the region from +121 to +139 CpG sites of the Piwil1 promoter CpG island, which was predicted to contain SOX2 binding sites. In addition, demethylation assays further demonstrated that DNA methylation indeed regulates Piwil1 expression during chicken spermatogenesis. Combined with transcription factor binding site prediction, we speculate that methylation influences the recruitment of corresponding transcription factors. Collectively, we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation.
分类号: S814
- 相关文献
作者其他论文 更多>>
-
CCPNet: Joining the pooling transformer and target context for medical image segmentation
作者:Yang, Yakun;Xue, Hongcheng;Wang, Longhe;Li, Lin;Liu, Xiangping;Feng, Chungang;Liu, Xiangping;Qu, Hao;Qu, Hao
关键词:Computer vision; Medical image segmentation; Pooling transformer; Target context; Tibial dyschondroplasia analysis
-
Production of neofemales by 17β-estradiol and YY super-male breeding in northern snakehead (Channa argus)
作者:Ou, Mi;Zhang, Yang;Luo, Qing;Chen, Kunci;Liu, Haiyang;Zhang, Xincheng;Fei, Shuzhan;Zhao, Jian;Ou, Mi;Zhang, Yang;Sun, Yuandong;Huang, Rong;Wang, Yaping;Yin, Jianxiong;Chen, Baixiang;Liu, Bingnan
关键词:Exogenous estrogen; Sex reversal; YY super-males; Snakehead
-
Impact of residual antibiotics on microbial decomposition of livestock manures in Eutric Regosol: Implications for sustainable nutrient recycling and soil carbon sequestration
作者:Fang, Linfa;Lakshmanan, Prakash;Su, Xiaoxuan;Shi, Yujia;Chen, Zheng;Zhang, Yu;Xiao, Ran;Chen, Xinping;Lakshmanan, Prakash;Wu, Junxi;Lakshmanan, Prakash;Lakshmanan, Prakash;Sun, Wei
关键词:Residual antibiotics; Livestock manure decomposition; Microbial community; Co -occurrence network; Enzyme activities
-
Comparative toxicity mechanisms of sulfoxaflor and lambda-cyhalothrin against Apolygus lucorum from enzymatic and transcriptomic perspectives: Efficient application of insecticides
作者:Xu, Lu;Liu, Baosheng;Xiao, Liubin;Zhang, Zhichun;Niu, Hongtao;Zhao, Dongxiao;Sun, Shuai;Guo, Huifang;Xu, Lu
关键词:Apolygus lucorum; Sulfoxaflor; Lambda-cyhalothrin; Transcriptome analysis; Toxicity difference; Enzyme activity
-
Effects of combined applications of S-nZVI and organic amendments on cadmium and arsenic accumulation in rice: Possible mechanisms and potential impacts on soil health
作者:Sun, Shuo;Zhang, Nan;Wang, Yanan;Zhang, Yang;Su, Shiming;Zeng, Xibai;Huang, Jiaqing;Wen, Jiong;Peng, Zhi
关键词:Cd & As immobilization; Combined application; Rice uptake Cd & As; Soil health; Soil health
-
A colorimetric and SERS dual-signal immunochromatography based on AuDTNB@PDA nanoparticles for the detection of triadimefon
作者:Dong, Sa;Zhu, Zixin;Shi, Qiuyun;Zhang, Yu;Feng, Jianguo;Wang, Yulong;Luo, Ruifeng
关键词:Triadimefon residue; Immunochromatographic strip; Colorimetric; SERS; Dual-signal
-
The ABF4-bHLH28-COMT5 module regulates melatonin synthesis and root development for drought tolerance in citrus
作者:Zhu, Jian;Wang, Yue;Xiao, Wei;Khan, Madiha;Fang, Tian;Dahro, Bachar;Liu, Ji-Hong;Jiang, Ling;Zhang, Yu;Ming, Ru-hong;Liu, Ji-Hong
关键词:
Poncirus trifoliata ; melatonin; drought tolerance; ABA; root development; transcriptional regulation