DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis

文献类型： 外文期刊

第一作者： Qiu, Lingling

作者： Qiu, Lingling;Xu, Lu;Chang, Guobin;Gu, Qixin;Bi, Yulin;Zhang, Yu;Lu, Wei;Ren, Lichen;Zhu, Pengfei;Mu, Yun;Zhang, Yang;Xu, Qi;Chen, Guohong;Liu, Xiangping;Wang, Hongzhi;Wang, Kehua

作者机构：

关键词： Chicken;DNA methylation;Piwil1;Spermatogenesis

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The P-element induced wimpy testis (Piwi) protein family is responsible for initiating spermatogenesis and maintaining the integrity of germ cells and stem cells, but little is known regarding its transcriptional regulation in poultry. Here, we characterized the methylation status of the Piwil1 promoter in five different spelmatogenic cell lines using direct bisulfite pyrosequencing and determined that methylation correlates negatively with germ cell type-specific expression patterns of piwil1. We demonstrated that methylation of the -148 CpG site, which is the predicted binding site for the transcription factors TCF3 and NRF1, was differentially methylated in different spermatogenic cells. This site was completely methylated in PGCs (primordial germ cells), but was unmethylated in round spermatids. A similar result was obtained in the region from +121 to +139 CpG sites of the Piwil1 promoter CpG island, which was predicted to contain SOX2 binding sites. In addition, demethylation assays further demonstrated that DNA methylation indeed regulates Piwil1 expression during chicken spermatogenesis. Combined with transcription factor binding site prediction, we speculate that methylation influences the recruitment of corresponding transcription factors. Collectively, we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation.

分类号： S814

  • 相关文献

[1]DNA methylation and NF-Y regulate Piwil1 expression during chicken spermatogenesis. Chang, Guobin,Chen, Rong,Xu, Lu,Ma, Teng,Wang, Hongzhi,Chen, Jing,Zhang, Yang,Li, Zhiteng,Wan, Fang,Guo, Xiaomin,Xu, Qi,Zhao, Wenming,Chen, Guohong,Chen, Rong.

[2]Folate supplementation modifies CCAAT/enhancer-binding protein alpha methylation to mediate differentiation of preadipocytes in chickens. Wen, Jie.

[3]Changes of host DNA methylation in domestic chickens infected with Salmonella enterica. Wang, Fei,Li, Jianchao,Li, Qinghe,Liu, Ranran,Zheng, Maiqing,Wang, Qiao,Wen, Jie,Zhao, Guiping.

[4]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[5]Effects of sub-chronic aluminum chloride on spermatogenesis and testicular enzymatic activity in male rats. Zhu, Y. Z.,Fu, Yang,Wang, J.,Song, M.,Li, M.,Li, Y. F.,Zhu, Y. Z.,Miao, L. G.,Sun, H.. 2014

[6]The low expression of Dmrt7 is associated with spermatogenic arrest in cattle-yak. Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Yan, Ping,Guo, Xian,Bao, Peng-Jia,Jin, Shuai,Wu, Xiao-Yun,Xiang, Lin.

[7]Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis. Wang, Yandi,Yu, Shuai,Feng, Yandi,Chu, Meigiang,Cui, Liantao,Li, Lan,Zhang, Pengfei,Shen, Wei,Min, Lingjiang,Wang, Yandi,Zhao, Yong,Yu, Shuai,Feng, Yandi,Chu, Meigiang,Cui, Liantao,Li, Lan,Shen, Wei,Min, Lingjiang,Zhao, Yong,Zhang, Hongfu,Kou, Xin.

[8]Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis). Zhang, Liyan,Zhao, Wen,Zhang, Liyan,Liu, Wanjun,Shao, Changwei,Zhang, Ning,Li, Hailong,Liu, Kun,Dong, Zhongdian,Qi, Qian,Chen, Songlin,Liu, Wanjun,Dong, Zhongdian,Qi, Qian.

[9]Characterization of a novel nm23 gene and its potential roles in gametogenesis in the prawn Macrobrachium rosenbergii (de Man, 1879) (Crustacea: Decapoda). Song, Ya-Nan,Lu, Cui-Yun,Chen, Jie,Qiu, Gao-Feng,Lu, Cui-Yun.

[10]Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable. Zhang, Weidong,Zhao, Yong,Chen, Liang,Yi, Bao,Tang, Xiangfang,Meng, Qingshi,Liu, Lei,Wang, Shukun,Zhang, Hongfu,Zhang, Weidong,Zhao, Yong,Zhang, Pengfei,Hao, Yanan,Yu, Shuai,Min, Lingjiang,Li, Lan,Ma, Dongxue,Shen, Wei. 2018

[11]Molecular cloning and characterization of hatching enzyme-like geneII (BmHELII) in the silkworm, Bombyx mori. Tang, Shunming,Wu, Jun,Zhao, Xinhui,Wang, Huanying,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie,Tang, Shunming,Qiu, Zhiyong,Shen, Xingjia,Guo, Xijie.

[12]Testes transcriptome profiles of the anadromous fish Coilia nasus during the onset of spermatogenesis. Zhou, Yan-Feng,Duan, Jin-Rong,Liu, Kai,Xu, Dong-Po,Zhang, Min-Ying,Fang, Di-An,Xu, Pao,Fang, Di-An,Xu, Pao.

[13]The effects of IGF-1 on mouse spermatogenesis using an organ culture method. Yao, Jianfei,Gao, Jun,Wang, Mingming,Li, Xiangdong,Zuo, Haiyang,Wang, Dong.

[14]Proteome analysis of silkworm, bombyx mori, larval gonads: Characterization of proteins involved in sexual dimorphism and gametogenesis. Chen, Jin-e,Li, Jian-ying,You, Zheng-ying,Jiang, Zhen-dong,Zhong, Bo-xiong,Chen, Jin-e,Li, Jian-ying,Liu, Li-li,Chen, Ming,Liang, Jian-she,Ma, Ying-ying,Zhang, Hua-rong.

[15]Widespread and evolutionary analysis of a MITE family Monkey King in Brassicaceae. Dai, Shutao,Hou, Jinna,Long, Yan,Wang, Jing,Li, Cong,Xiao, Qinqin,Jiang, Xiaoxue,Zou, Xiaoxiao,Zou, Jun,Meng, Jinling,Hou, Jinna. 2015

[16]Genome-wide high-resolution mapping of DNA methylation identifies epigenetic variation across embryo and endosperm in Maize (Zea may). Wang, Pengfei,Ma, Chuanxi,Wang, Pengfei,Xia, Han,Zhang, Ye,Zhao, Shuzhen,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Li, Aiqin,Wang, Xingjun. 2015

[17]Synonymous Codon Usage Bias in the Plastid Genome is Unrelated to Gene Structure and Shows Evolutionary Heterogeneity. Qi, Yueying,Xu, Wenjing,Xing, Tian,Zhao, Mingming,Yan, Li,Xia, Guangmin,Wang, Mengcheng,Li, Nana. 2015

[18]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[19]Study on DNA Cytosine Methylation of Cotton (Gossypium hirsutum L.) Genome and Its Implication for Salt Tolerance. ZHAO Yun-lei,YU Shu-xun,YE Wu-wei,WANG Hong-mei,WANG Jun-juan,FANG Bao-xing. 2010

[20]Induced Pib Expression and Resistance to Magnaporthe grisea are Compromised by Cytosine Demethylation at Critical Promoter Regions in Rice. Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Li, Yuan,Xia, Qiong,Wu, Ying,Xu, Chunming,Liu, Bao,Kou, Hongping,Wang, Dan,Lin, Xiuyun,Xing, Shaochen. 2011

作者其他论文 更多>>

微信小程序