Identification of a novel Aleutian mink disease virus B-cell epitope using a monoclonal antibody against VP2 protein

文献类型: 外文期刊

第一作者: Yi, Li

作者: Yi, Li;Cheng, Yuening;Zhang, Miao;Cao, Zhigang;Tong, Mingwei;Cheng, Shipeng;Yan, Xijun

作者机构:

关键词: Aleutian mink disease virus;VP2 protein;Monoclonal antibody;B-cell epitope

期刊名称:VIRUS RESEARCH ( 影响因子:3.303; 五年影响因子:3.445 )

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收录情况: SCI

摘要: Aleutian mink disease virus (AMDV) is a parvovirus that causes an immune complex-mediated disease in minks. Capsid protein VP2 is a major structural viral protein and can be used to diagnose AMDV. In this study, a specific monoclonal antibody, 1M13, was produced against the AMDV VP2 protein (amino acids 291-502). A linear VP2-protein epitope was identified by subjecting a series of partially overlapping synthesized peptides to be enzyme-linked immunosorbent assay (ELISA) analysis. The results indicated that (386)HLQQNFSTRYIYD(398) was the minimal linear epitope that could be recognized by mAb 1M13. ELISA assays revealed that mink anti-AMDV sera could also recognize the minimal linear epitope. Sequence alignments demonstrated that the linear epitope is highly conserved among AMDV strains except H-386 and is less conserved among Raccoon dog amdovirus, Gray fox amdovirus, Red fox amdovirus, Bat parvovirus and Mink enteritis parvovirus. Taken together, the generation of this VP2-specific mAb with a defined linear peptide epitope may have potential applications in the development of suitable diagnostic techniques for AMDV. (C) 2016 Elsevier B.V. All rights reserved.

分类号: R37

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