A single amino acid mutation of OsSBEIIb contributes to resistant starch accumulation in rice
文献类型: 外文期刊
第一作者: Yang, Ruifang
作者: Yang, Ruifang;Bai, Jianjiang;Fang, Jun;Piao, Zhongze;Wang, Ying;Lee, Gangseob
作者机构:
关键词: resistant starch;sbe3-rs;rice;starch branching enzyme;complementation assay;Jiangtangdao1
期刊名称:BREEDING SCIENCE ( 影响因子:2.086; 五年影响因子:2.632 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Foods rich in resistant starch can help prevent various diseases, including diabetes, colon cancers, diarrhea, and chronic renal and hepatic diseases. Variations in starch biosynthesis enzymes could contribute to the high content of resistant starch in some cultivars of rice (Oryza sativa L.). Our previously published work indicated that the sbe3-rs gene in the rice mutant line, 'Jiangtangdao1' was a putative allele of the rice starch branching enzyme gene SBEIIb (previously known as SBE3); sbe3-rs might control the biosynthesis of the high resistant starch content in the rice line. Biomolecular analysis showed that the activity of SBEs was significantly lower in soluble extracts of immature seeds harvested from 'Jiangtangdao1' 15 days after flowering than in the extracts of the wild-type rice line 'Huaqingdao'. We performed gene complementation assays by introducing the wild-type OsSBEIIb into the sbe3-rs mutant 'Jiangtangdao1'. The genetically complemented lines demonstrated restored seed-related traits. The structures of endosperm amylopectin and the morphological and physicochemical properties of the starch granules in the transformants recovered to wild-type levels. This study provides evidence that sbe3-rs is a novel allele of OsSBEIIb, responsible for biosynthesis of high resistant starch in 'Jiangtangdao1'.
分类号: S33
- 相关文献
作者其他论文 更多>>
-
Continuous monitoring the Queen loss of honey bee colonies
作者:Lu, Yuntao;Liu, Shengping;Hong, Wei;Hong, Wei;Fang, Yu;Wang, Ying;Liu, Zhenguo;Wang, Hongfang;Xu, Baohua;Lu, Chuanqi
关键词:Honeybee; Queen loss; Apis mellifera; Colony health; Colony state; Colony monitoring system
-
Pre-harvest treatments: A different insight into preservation of strawberries
作者:Xu, Jingcheng;Wang, Ying;Yin, Danping;Lin, Tingwei;Tao, Yujia;Liu, Shudong;Qi, Huijuan;Gao, Xianyi;Jiang, Jingyong;Xu, Jingcheng;Chai, Lin
关键词:Strawberry; pre-harvest treatments; post-harvest quality; storability
-
RNA-seq-based transcriptome profiling of early fruit development in Chieh-qua and analysis of related transcription factors
作者:Du, Xuan;Liu, Na;Lu, Panling;Wang, Ying;Tian, Shoubo;Lu, Bo;Zhang, Zhaohui
关键词:Transcriptome; Early fruit development; Chieh-qua; Transcription factors; MYB_superfamily
-
A self-adaptive parallel image stitching algorithm for unmanned aerial vehicles in edge computing environments
作者:Xu, Xin;Zhang, Li;Yue, Jibo;Zhong, Heming;Wang, Ying;Qiao, Hongbo;Liu, Jie;Lu, Yanhui
关键词:UAV remote sensing; panoramic stitching; multi-core CPU; multi process; edge computing
-
Fermented natto powder alleviates obesity by regulating LXR pathway and gut microbiota in obesity rats
作者:Sun, Ruiyue;Niu, Honghong;Sun, Mubai;Hua, Mei;Miao, Xinyu;Su, Ying;Wang, Jinghui;Li, Da;Sun, Ruiyue;Li, Yueqiao;Wang, Ying
关键词:Bacillus subtilis natto; natto powder; soybean powder; obesity; cholesterol metabolism; gut microbiota
-
Excavation of Genes Response to Heat Resistance by Transcriptome Analysis in Bottle Gourd (Lagenaria siceraria (Mol.) Standl.)
作者:Wang, Min;Liu, Wenrui;Peng, Qingwu;Shi, Shaoqi;Jiang, Biao;Lin, Yu'e;Yang, Songguang;Wang, Min;Liu, Wenrui;Shi, Shaoqi;Cao, Liqin;Jiang, Biao;Zhao, Tianyue;Cui, Xiaojuan;Yang, Songguang;Wang, Ying
关键词:bottle gourd; RNA-Seq; DEGs; MAPK; bHLH; heat stress
-
The role of uterus mitochondrial function in high-fat diet-related adverse pregnancy outcomes and protection by resveratrol
作者:Yang, Xizi;Hu, Ruizhi;Yao, Liping;Zhang, Wentao;Shi, Mingkun;Gong, Jiatai;Li, Yanli;Yan, Jiahao;Wang, Ying;Zhang, Qianjin;Fan, Zhiyong;He, Xi;He, Jianhua;Wu, Shusong;Yuan, Xupeng;He, Ziyu;Hou, De-Xing;Zhang, Hongfu;Chen, Liang
关键词: