A single amino acid mutation of OsSBEIIb contributes to resistant starch accumulation in rice
文献类型: 外文期刊
第一作者: Yang, Ruifang
作者: Yang, Ruifang;Bai, Jianjiang;Fang, Jun;Piao, Zhongze;Wang, Ying;Lee, Gangseob
作者机构:
关键词: resistant starch;sbe3-rs;rice;starch branching enzyme;complementation assay;Jiangtangdao1
期刊名称:BREEDING SCIENCE ( 影响因子:2.086; 五年影响因子:2.632 )
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收录情况: SCI
摘要: Foods rich in resistant starch can help prevent various diseases, including diabetes, colon cancers, diarrhea, and chronic renal and hepatic diseases. Variations in starch biosynthesis enzymes could contribute to the high content of resistant starch in some cultivars of rice (Oryza sativa L.). Our previously published work indicated that the sbe3-rs gene in the rice mutant line, 'Jiangtangdao1' was a putative allele of the rice starch branching enzyme gene SBEIIb (previously known as SBE3); sbe3-rs might control the biosynthesis of the high resistant starch content in the rice line. Biomolecular analysis showed that the activity of SBEs was significantly lower in soluble extracts of immature seeds harvested from 'Jiangtangdao1' 15 days after flowering than in the extracts of the wild-type rice line 'Huaqingdao'. We performed gene complementation assays by introducing the wild-type OsSBEIIb into the sbe3-rs mutant 'Jiangtangdao1'. The genetically complemented lines demonstrated restored seed-related traits. The structures of endosperm amylopectin and the morphological and physicochemical properties of the starch granules in the transformants recovered to wild-type levels. This study provides evidence that sbe3-rs is a novel allele of OsSBEIIb, responsible for biosynthesis of high resistant starch in 'Jiangtangdao1'.
分类号: S33
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