ENZYME-CATALYZED EXTRACTION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDES FROM PHELLINUS IGNIARIUS

文献类型： 外文期刊

第一作者： Xu, Yan

作者： Xu, Yan;Zhao, Xing-Yu;Cao, Hui;Sheng, Sheng;Wang, Jun;Wu, Fu-An;Xu, Yan;Sheng, Sheng;Wang, Jun;Wu, Fu-An

作者机构：

关键词： ABTS free-radical scavenging activity;DPPH radical scavenging activity;Enzyme-catalyzed extraction;Phellinus igniarius polysaccharides;Response surface methodology;Total reducing power

期刊名称：CURRENT TOPICS IN NUTRACEUTICAL RESEARCH （ 影响因子：0.416； 五年影响因子：0.536 ）

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年卷期：

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收录情况： SCI

摘要： A new process for the enzyme-catalyzed extraction of polysaccharides from Phellinus igniarius was developed Parameters included the pH value, temperature, enzyme dosage and extraction time. The effects of these parameters on extraction yields of P. igniarius polysaccharides (PIPs) were investigated and the optimal process was further determined by using response surface methodology (RSM). Assays to determine the total reducing power, ABTSfree-radical scavenging activity and DPPH radical scavenging activity were then used to determine the antioxidant activity of the extracted PIPs. The results showed that the optimal conditions for enzyme-catalyzed extraction included a pH value of 5.79, a reaction time of 1.5 h, an extraction temperature of 55.02 degrees C and enzyme dosage of 3.04%. Compared with conventional hot-water extraction, the extraction yield of PIPs was increased by 49.5%. In addition, the antioxidant activity of the PIPs was stronger than that of other fungus polysaccharides from Agrocybe aegirit, Hericium erinaceus, Flammulina velutiper and Lentinus edodes. When the PIP concentration was 4 g/L, total reducing power was 0.643 +/- 0.012, ABTS free-radical scavenging activity was 95.40 +/- 0.16% and DPPH radical scavenging activity was 69.08 +/- 3.03%. Thus, the development of an enzyme-catalyzed method for extracting PIPs from P igniarius was found to be feasible and to show high extraction efficacy, and the PIPs displayed good antioxidant activity.

分类号： R1

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