Cloning and Characterization of a Nanog Pseudogene in Sika Deer (Cervus nippon)

文献类型： 外文期刊

第一作者： Wang, Datao

作者： Wang, Datao;Guo, Qianqian;Ba, Hengxing;Li, Chunyi;Wang, Datao;Li, Chunyi

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关键词： deer antler;antler stem cell;Nanog;nuclear protein;stem cell marker

期刊名称：DNA AND CELL BIOLOGY （ 影响因子：3.311； 五年影响因子：3.557 ）

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收录情况： SCI

摘要： Nanog plays a crucial role in the maintenance of stem cell pluripotency. Annual full regeneration of deer antlers has been shown to be a stem cell-based process, and antler stem cells (ASCs) reportedly express Nanog. In the present study, we found that Nanog RNA expressed by ASCs was a pseudogene (Nanog-ps). The coding sequence of Nanog-ps was 93.1% homologous to that of bovine Nanog, but with two missing nucleotides after position 391. Deletion of the two nucleotides in Nanog-ps resulted in a frame-shift mutation, suggesting that Nanog-ps would not encode a normal Nanog protein. Overexpression of Nanog-ps failed to affect downstream genes of Nanog or to enhance cell proliferation in the ASCs. However, this pseudogene was transcribed in the ASCs and encoded a nuclear protein; the expression levels of Nanog-ps were also related to the degree of stemness in antler cells. Here, we reported this pseudogene, because it could serve as a useful marker for identifying ASCs and evaluating the degree of their stemness.

分类号： Q343

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