Single-cell RNA-seq identifies a PD-1(hi) ILC progenitor and defines its development pathway

文献类型： 外文期刊

第一作者： Yu, Yong

作者： Yu, Yong;Tsang, Jason C. H.;Wang, Cui;Clare, Simon;Wang, Juexuan;Chen, Xi;Brandt, Cordelia;Kane, Leanne;Campos, Lia S.;Teichmann, Sarah A.;Dougan, Gordon;Liu, Pentao;Tsang, Jason C. H.;Tsang, Jason C. H.;Wang, Cui;Lu, Liming;Belz, Gabrielle T.;Belz, Gabrielle T.;McKenzie, Andrew N. J.;Teichmann, Sarah A.;Dougan, Gordon

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期刊名称：NATURE （ 影响因子：49.962； 五年影响因子：54.637 ）

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收录情况： SCI

摘要： Innate lymphoid cells (ILCs) functionally resemble T lymphocytes in cytotoxicity and cytokine production but lack antigen-specific receptors, and they are important regulators of immune responses and tissue homeostasis(1,2). ILCs are generated from common lymphoid progenitors, which are subsequently committed to innate lymphoid lineages in the alpha-lymphoid progenitor, early innate lymphoid progenitor, common helper innate lymphoid progenitor and innate lymphoid cell progenitor compartments(3-8). ILCs consist of conventional natural killer cells and helper-like cells (ILC1, ILC2 and ILC3)(9). Despite recent advances(1,2,10), the cellular heterogeneity, developmental trajectory and signalling dependence of ILC progenitors are not fully understood. Here, using single-cell RNA-sequencing (scRNA-seq) of mouse bone marrow progenitors, we reveal ILC precursor subsets, delineate distinct ILC development stages and pathways, and report that high expression of programmed death 1 (PD-1(hi)) marked a committed ILC progenitor that was essentially identical to an innate lymphoid cell progenitor. Our data defined PD-1(hi)IL-25R(hi) as an early checkpoint in ILC2 development, which was abolished by deficiency in the zinc-finger protein Bcl11b but restored by IL-25R overexpression. Similar to T lymphocytes, PD-1 was upregulated on activated ILCs. Administration of a PD-1 antibody depleted PD-1(hi) ILCs and reduced cytokine levels in an influenza infection model in mice, and blocked papain-induced acute lung inflammation. These results provide a perspective for exploring PD-1 and its ligand (PD-L1) in immunotherapy, and allow effective manipulation of the immune system for disease prevention and therapy.

分类号： N

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