Production of Proinsulin in Marker-Free Transgenic Tobacco Plants Using CRE/loxP System

文献类型： 外文期刊

第一作者： Zheng, L.

作者： Zheng, L.;Wang, Y.;Wan, S. B.;Bi, Y. P.;Zheng, L.;Peng, Z. Y.;Wang, Y.;Bian, F.;Wan, S. B.;Bi, Y. P.;Zheng, L.;Peng, Z. Y.;Wang, Y.;Bian, F.;Wan, S. B.;Bi, Y. P.;Jiao, Q. Q.;Qu, S. J.;Wan, S. B.;Bi, Y. P.

作者机构：

关键词： Nicotiana tabacum;diabetes;PROINSULIN;CRE/loxP;marker-free;transgenic tobacco plant

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The demand for INSULIN is increasing rapidly along with the increased number of diabetic patients. Using the CRE/loxP system, we developed a selective marker-free system without crossing to produce PROINSULIN in transgenic plant. In frame of this approach, the induced promoter pRD29A was isolated from Arabidopsis. The CRE recombinase gene was placed under the control of pRD29A between two loxP recombination sites together with the selective NPTII gene. Furthermore, the binary vector with CRE recombinase and PROINSULIN was constructed and introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. Gene excision was used to remove the sequence between the two loxP sites at the presence of 200 mM NaCl. PCR analysis showed that self-excision occurred in several T-0 transgenic plants. Transgenic plants without any marker gene successfully expressed PROINSULIN. This auto-excision strategy provides efficient means of removing the selectable marker gene from transgenic plants. It is an efficient method for producing bio-safe recombinant protein and other valuable substances in plants.

分类号： Q94

  • 相关文献

[1]Transgenic Expression and Identification of Recombinant Human Proinsulin in Peanut. Zheng Ling,Wang Yu,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Zheng Ling,Wang Yu,Bian Fei,Wan Shu-Bo,Peng Zhen-Ying,Bi Yu-Ping,Jiao Qi-Qing,Qu Shu-Jie,Wan Shu-Bo,Bi Yu-Ping. 2016

[2]Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun,Zou, Xiuping,Peng, Aihong,Xu, Lanzhen,Liu, Xiaofeng,Lei, Tiangang,Yao, Lixiao,He, Yongrui,Chen, Shanchun. 2013

[3]Green fluorescent protein as a vital elimination marker to easily screen marker-free transgenic progeny derived from plants co-transformed with a double T-DNA binary vector system. Chen, SB,Li, XG,Liu, X,Xu, HL,Meng, K,Xiao, GF,Wei, XL,Wang, F,Zhu, Z. 2005

[4]Production of marker-free transgenic rice expressing tissue-specific Bt gene. Qiu, Chengxiang,Sangha, Jatinder Singh,Zhou, Zhiyun,Yin, Ao,Gu, Keyu,Tian, Dongsheng,Yin, Zhongchao,Song, Fengshun,Yang, Jianbo. 2010

[5]Excision of a selectable marker in transgenic lily (Sorbonne) using the Cre/loxP DNA excision system. Li, Sh.,Du, Y. -P.,Wang, Zh. -X.,Jia, G. -X.,Li, Sh.,Wu, Zh. -Y.,Huang, C. -L.,Zhang, X. -H.. 2013

[6]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[7]Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase. Cao, MX,Huang, JQ,Yao, QH,Liu, SJ,Wang, CL,Wei, ZM. 2006

[8]Marker-free, tissue-specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants. Qi, Yongbin,Chen, Lei,Jin, Qingsheng,Zhang, Xiaoming,Qi, Yongbin,He, Zuhua,He, Xiuling,He, Zuhua. 2013

[9]Generation of double-virus-resistant marker-free transgenic potato plants. Bai, Yunfeng,Guo, Zhihua,Wang, Xiaoqi,Bai, Dongmei,Zhang, Weifeng. 2009

[10]Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation. Feng, Dan,Wang, Yanwei,Wu, Jinxia,Lu, Tiegang,Zhang, Zhiguo. 2017

[11]Cre/lox-mediated Marker Gene Excision in Elite Indica Rice Plants Transformed with Genes Conferring Resistance to Lepidopteran Insects. Chen, SB,Liu, X,Peng, HY,Gong, WK,Wang, R,Wang, F,Zhu, Z.

[12]High level resistance to Turnip mosaic virus in Chinese cabbage (Brassica campestris ssp pekinensis (Lour) Olsson) transformed with the antisense NIb gene using marker-free Agrobacterium tumefaciens infiltration. Yu Zhandong,Zhao Shuangyi,He Qiwei.

[13]A chemical-inducible Cre-LoxP system allows for elimination of selection marker genes in transgenic apricot. Petri, Cesar,Lopez-Noguera, Sonia,Wang, Hong,Garcia-Almodovar, Carlos,Alburquerque, Nuria,Burgos, Lorenzo,Wang, Hong.

[14]Severe hypertriglyceridemia and hypercholesterolemia accelerating renal injury: a novel model of type 1 diabetic hamsters induced by short-term high-fat/high-cholesterol diet and low-dose streptozotocin. He, Liang,Fu, Xin,Huang, Mingshu,Hao, Lili,Li, Rui,Li, Rui. 2015

[15]Enhancement of the Pharmacological Efficacy of FGF-21 by Genetic Modification and PEGylation. Ye, Xianlong,Qi, Jianying,Ren, Guiping,Zhu, Shenglong,Wu, Yunzhou,Yu, Dan,Liu, Mingyao,Li, Deshan,Sun, Guopeng,Zhao, Jingzhuang,Li, Deshan. 2013

[16]Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level. Huang, Qingde,Xu, Jiqu,Deng, Qianchun,Huang, Fenghong,Yang, Wei,Fu, Juan,Yu, Miao,Wang, Di,Yao, Ping,Liu, Liegang,Yang, Wei,Fu, Juan,Yu, Miao,Wang, Di,Yao, Ping,Liu, Liegang. 2012

[17]Protective effect of Schisandrae chinensis oil on pancreatic beta-cells in diabetic rats. An, Liping,Wang, Chunmei,Han, Xiao,Xu, Guangyu,Yuan, Guangxin,Li, Hongyu,Sheng, Yu,Wang, Manli,Sun, Jingbo,Zhan, Jinzhuo,Sun, Hui,Li, Na,Du, Peige,Wang, Yingping,Fan, Meizhen,Ding, Fuxiang. 2015

[18]Antibacterial Activity of Polyphenols: Structure-Activity Relationship and Influence of Hyperglycemic Condition. Xie, Yixi,Chen, Jing,Xiao, Aiping,Liu, Liangliang,Xie, Yixi. 2017

[19]Therapeutic Strategies of Plant-derived Compounds for Diabetes Via Regulation of Monocyte Chemoattractant Protein-1. Czemplik, Magdalena,Kulma, Anna,Szopa, Jan,Wang, Yu Fu,Szopa, Jan,Szopa, Jan.

[20]Comparative Evaluation of Quercetin, Isoquercetin and Rutin as Inhibitors of alpha-Glucosidase. Li, Yan Qin,Zhou, Feng Chao,Gao, Fei,Bian, Jun Sheng,Shan, Fang. 2009

作者其他论文 更多>>

微信小程序