Molecular cloning of chicken IL-7 and characterization of its antiviral activity against IBDV in vivo

文献类型： 外文期刊

第一作者： Huo, Shanshan

作者： Huo, Shanshan;Wang, Liyue;Zhang, Yonghong;Zhang, Jianlou;Zuo, Yuzhu;Cui, Dan;Zhong, Fei;Huo, Shanshan;Wang, Liyue;Zhang, Yonghong;Zhong, Fei;Xu, Jian;Li, Xiujin

作者机构：

关键词： chicken interleukin-7;eukaryotic expression;IBDV;prevention;treatment

期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）

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收录情况： SCI

摘要： Mammalian interleukin-7 (IL-7) is able to stimulate lymphocyte proliferation and maturation, and reverse immunosuppression. However, whether poultry IL-7 has similar functions remains unclear. Chicken infectious bursal disease virus (IBDV) causes serious immunosuppression in chicken due to virus-induced immune disorder. Whether chicken IL-7 (chIL-7) has the ability to restore the immunity during IBDV-induced immunosuppression is not clear. To test this, we amplified chIL-7 gene by RT-PCR, prepared recombinant chIL-7 using HEK293T cells and treated the chicken with the chIL-7 prior to IBDV infection. Our results indicate that chIL-7 promoted mouse B cell proliferation in vitro, and significantly reduced virus titer in bursal tissue and chicken morbidity of IBDV-infected chicken. Mechanically, chIL-7 induced chicken lymphocyte proliferation and interferon-gamma production, but down-regulated TGF-beta expression, suggesting that chIL-7 has the ability to reverse IBDV-induced immunosuppression and might be a potential therapeutic agent for prevention and treatment of infectious bursal disease.

分类号： S8

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