In vitro observation of the stage conversion of transgenic Toxoplasma gondii RH strain expressing dual fluorescent proteins

文献类型： 外文期刊

第一作者： Song, Qijun

作者： Song, Qijun;Sun, Ximeng;Ji, Yongsheng;Yan, Xinlei;Zou, Jun;Zhao, Shiyun;Suo, Xun;Liu, Xianyong;Zhu, Xingquan

作者机构：

关键词： Toxoplasma gondii;transgenic;fluorescent proteins;stage conversion;asynchronous development

期刊名称：ACTA PARASITOLOGICA （ 影响因子：1.44； 五年影响因子：1.335 ）

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收录情况： SCI

摘要： Toxoplasma gondii converts from tachyzoites to bradyzoites after acute infection and thus survives the attack of the host immune responses. In this study, we observed the conversion of tachyzoites to bradyzoites in cell cultures using a transgenic T. gondii RH strain. The transgenic parasites continuously express yellow fluorescent protein (YFP) but only express red fluorescent protein (RFP) at the bradyzoite stage. Red fluorescent bradyzoite-containing cysts were found in transgenic parasite infected cells cultured with atmospheric CO2 supply, indicating the successful induction of the stage conversion. In cell culture with alkalic medium (pH 8.1) and atmospheric CO2 supply, only part of the YFP-expressing parasites in a cyst express RFP marker, suggesting the asynchronous development of T. gondii in vitro. This study provides a possibility for further studies of the gene expression profile during stage conversion and the genes involved.

分类号： R38

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