Heat-induced apoptosis and gene expression in bovine mammary epithelial cells

文献类型： 外文期刊

第一作者： Hu, Han

作者： Hu, Han;Wang, Jiaqi;Gao, Haina;Li, Songli;Zhang, Yangdong;Zheng, Nan;Hu, Han;Wang, Jiaqi;Gao, Haina;Li, Songli;Zhang, Yangdong;Zheng, Nan;Hu, Han;Wang, Jiaqi;Gao, Haina;Li, Songli;Zhang, Yangdong;Zheng, Nan

作者机构：

关键词： bovine mammary epithelial cell;heat shock proteins;heat stress

期刊名称：ANIMAL PRODUCTION SCIENCE （ 影响因子：1.533； 五年影响因子：1.779 ）

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收录情况： SCI

摘要： The objective of this study was to identify the apoptosis and cell-defence response of bovine mammary epithelial cells under heat stress (HS). Cells were exposed to either 38 degrees C or 42 degrees C for 0.5, 1, 3, 5, 8, or 12 h, and the transcription of heat shock proteins (Hsps), Bcl-2 family, caspases and apoptosis-regulated genes were quantified by quantitative real-time polymerase chain reaction. Caspase-3, -7 and -8 were markedly upregulated by HS and the peak gene abundance appeared at 5 h. However, the same family numbers, caspase-6 and -9 were sustained downregulated in HS. The expression of anti-apoptotic gene Bcl-2, Bcl-2A and Mcl-1 increased sharply in HS but returned to pre-HS levels after 8 h. The pro-apoptotic genes: Bax, Bak and Bid were downregulated during HS. The striking changes of signalling factors of apoptosis: tumour necrosis factor receptor, p53, Apaf-1 was upregulated, and Fas was downregulated in HS. Stress proteins Hsp genes (hsp27, hsp70 and hsp90) were generally increased at 42 degrees C and this was especially apparent for hsp70 transcription as it was increased 14-fold at 1 h. Simultaneously, HS induced cell apoptosis, and the peak of apoptosis rate appeared at 3 and 5 h, which were assessed by flow cytometry. Our results suggest that HS induces cell apoptosis, disturbs the normal biological activity, and aroused intracellular thermotolerance responses of bovine mammary epithelial cells.

分类号： S`S8

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