CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae

文献类型: 外文期刊

第一作者: Fang, Xiaomin

作者: Fang, Xiaomin;Zhao, Weimin;Xu, Jie;Tu, Feng;Wang, Xuemin;Li, Bixia;Fu, Yanfeng;Ren, Shouwen

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关键词: Pig;CYP1A1;Mycoplasma hyopneumoniae;Inflammatory response

期刊名称:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ( 影响因子:3.636; 五年影响因子:3.654 )

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收录情况: SCI

摘要: Mycoplasmal pneumonia is a lung infection disease caused by Mycoplasma hyopneumoniae in swine. We previously reported that Cytochrome P450 1A1 (CYP1A1) expression was significantly downregulated in pigs infected with M. hyopneumoniae compared to the healthy controls. In this study, pulmonary alveolar macrophage (PAM) cell lines with CYP1A1 overexpression or siRNA-mediated CYP1A1 silencing were used to explore the biological function and regulatory mechanism of CYP1A1 gene expression changed on the inflammatory response of pigs infected with M. hyopneumoniae. The results showed that the cells overexpressing CYP1A1 infected with M. hyopneumoniae led to a rapid increase in PPAR-gamma expression, which resulted in decreasing the levels of several inflammatory cytokines including IL-1 beta, IL-6, IL-8 and TNF-alpha. On the contrary, this effect was just opposite in CYP1A1-RNAi cells infected with M. hyopneumoniae. We suggest that CYP1A1 suppress the inflammatory response caused by M. hyopneumoniae infection, via PPAR-gamma signaling pathway in pigs. (C) 2016 Elsevier Ltd. All rights reserved.

分类号: R392

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