The E3 ubiquitin ligase TaGW2 facilitates TaSnRK1γ and TaVPS24 degradation to enhance stripe rust susceptibility in wheat

文献类型： 外文期刊

第一作者： Li, Shumin

作者： Li, Shumin;Feng, Wenxuan;Mao, Hude;Li, Tian;Hao, Chenyang;Zhang, Xueyong;Zhang, Peiyin;Wang, Xuemin;Zhang, Yifang;Chen, Bin;Liu, Yuling;Zhan, Gangming;Kang, Zhensheng

作者机构：

关键词： wheat; strip rust; TaGW2; ubiquitination; TaSnRK1 gamma; TaVPS24

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）

ISSN： 1467-7644

年卷期： 2025 年 23 卷 3 期

页码：

收录情况： SCI

摘要： Wheat stripe rust, caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), threatens global wheat production, and therefore discovering genes involved in stripe rust susceptibility is essential for balancing yield with disease resistance in sustainable breeding strategies. Although TaGW2 is well known to negatively regulate wheat kernel size and weight, its role in stress response remains unclear. Here, we found that TaGW2 transcription levels increased following inoculation with Pst or treatment with flg22 or chitin. TaGW2 knockdown lines showed enhanced resistance to multiple Pst races, while TaGW2 overexpression reduced host defence response, promoted Pst growth and development and increased wheat susceptibility to Pst. Additionally, TaGW2 could mediate the ubiquitination and degradation of both TaSnRK1 gamma and TaVPS24 via the 26S proteasome pathway. Silencing TaSnRK1 gamma or TaVPS24 in wheat increased sensitivity to Pst, whereas overexpressing either gene enhanced wheat defence response, indicating that TaSnRK1 gamma and TaVPS24 act as positive regulators of Pst resistance. This study reveals a previously unrecognized mechanism inhibiting plant immunity to Pst through TaGW2-mediated ubiquitination and degradation of TaSnRK1 gamma and TaVPS24. This work also provides crucial genetic resources for breeding high-yield, stripe rust-resistant wheat varieties.

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