Metabolic Changes of Caffeine in Tea Plant (Camellia sinensis (L.) O. Kuntze) as Defense Response to Colletotrichum fructicola

文献类型: 外文期刊

第一作者: Wang, Yu-Chun

作者: Wang, Yu-Chun;Qian, Wen-Jun;Xiao, Bin;Yang, Ya-Jun;Wang, Yu-Chun;Qian, Wen-Jun;Li, Na-Na;Hao, Xin-Yuan;Wang, Lu;Wang, Xin-Chao;Yang, Ya-Jun

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关键词: Theaceae;disease resistance;tea polyphenols;catechins;Colletotrichum

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

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收录情况: SCI

摘要: Tea plant (Camellia sinensis) is one of the most economically valuable crops in the world. Anthracnose can affect the growth of leaves and cause serious yield losses of tea. Tea plants are rich in secondary metabolites; however, their roles in resistance to anthracnose are unclear. Herein we compared the contents of total phenolics, catechins, and caffeine in two cultivars with different resistances to anthracnose during Colletotrichum fructicola infection. (-)-Epigallocatechin-3-gallate (EGCG), (+)-catechin (C), caffeine, and critical regulatory genes were induced in C. fructicola-resistant tissues. In vitro antifungal tests showed that caffeine more strongly inhibited mycelial growth than tea polyphenols and catechins. Both electron microscopy and bioactivity analysis results showed that caffeine can affect mycelial cell walls and plasma membranes. Through promoter sequences analysis, a number of stress response-related cis-acting elements were identified in S-adenosylmethionine synthetase and tea caffeine synthase. These results demonstrated that ()-EGCG, (+)-C, and caffeine may be involved in the resistance of tea plants to anthracnose.

分类号: R15`S

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