Molecular characterization and expression analysis of toll-like receptor 2 in response to bacteria in silvery pomfret intestinal epithelial cells

文献类型： 外文期刊

第一作者： Gao, Quanxin

作者： Gao, Quanxin;Zhang, Chenjie;Min, Minghua;Peng, Shiming;Shi, Zhaohong;Xiao, Yingping

作者机构：

关键词： Toll-like receptor 2;Silvery pomfret;Molecular cloning;Expression modulation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Toll-like receptor 2 (TLR2) has been shown to play a crucial role in the host defense of pathogenic microbes in innate immunity. In this study, the full-length cDNA of TLR2 in silvery pomfret (Pampus argenteus) was cloned by homology cloning and the rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of TLR2 was 2932 bp, containing an open reading frame (ORF) of 2469 bp encoding 822 amino acids. A multiple alignment analysis of the silvery pomfret TLR2 protein-coding sequence with other known TLR2 sequences from Oplegnathus fasciatus, Epinephelus coioides, Larimichthys crocea, Miichthys miiuy, Oreochromis niloticus, Paralichthys olivaceus, Trematomus bernacchii, Sparta aurata, and Chionodraco hamatus exhibited a high degree of homology of 78.83%, 75.91%, 74.21%, 74.94%, 71.95%, 72.57%, 73.68%, 75%, and 72.52 respectively, between these fish. Analysis of the TLR2 domain structures indicated that TLR2 from the silvery pomfret has the typical structural features of proteins that belong to the TLR family, including one transmembrane domain, eleven leucine-rich repeats (LRRs), and one Toll/IL-1 receptor homology domain (TIR). In vitro immunostimulation experiments revealed that Lactobacillus plantarum and Clostridium butyricum induce high levels of TLR2 mRNA and protein expression, but they induce only moderate levels of IL-8 and TNF-alpha production compared to Vibrio anguillarum. This suggests that TLR2 might play a vital role in the L. plantarum and C butyricum-mediated immune response. In contrast, V. anguillarum significantly increased the secretion of IL-8 and TNF-alpha and induced cell apoptosis and necrosis. Due to the lower expression of TLR2 and higher levels of IL-8 and TNF-alpha induced by V. anguillarum, we hypothesize that a V anguillarum infection is independent of the TLR2-induced production of pro-inflammatory cytokines. These results indicate that TLR2 may be involved in molecular interactions between the host and commensal bacteria, that exist in the silvery pomfret intestinal tract. (C) 2016 Published by Elsevier Ltd.

分类号： S9

  • 相关文献

[1]Cloning, characterization, and function of MyD88 in silvery pomfret (Pampus argenteus) in response to bacterial challenge. Gao, Quanxin,Yin, Fei,Zhang, Chenjie,Yue, Yanfeng,Sun, Peng,Min, Minghua,Peng, Shiming,Shi, Zhaohong,Lv, Jing.

[2]TLR2 mediates autophagy through ERK signaling pathway in Mycoplasma gallisepticum-infected RAW264.7 cells. Lu, Ziyin,Xie, Daoyuan,Chen, Ying,Tian, Erjie,Muhammad, Ishfaq,Chen, Xueping,Miao, Yusong,Hu, Wanjun,Wu, Ziyong,Ni, Huili,Li, Jichang,Xin, Jiuqing,Li, Yuan.

[3]Molecular characterization and expression analysis of MHC class II alpha and beta genes in large yellow croaker (Pseudosciaena crocea). Yu, Suhong,Ao, Jingqun,Chen, Xinhua,Yu, Suhong,Yu, Suhong. 2010

[4]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[5]Molecular and functional characterization of a novel stefin analogue in large yellow croaker (Pseudosciaena crocea). Li, Shuying,Yang, Zhijun,Ao, Jingqun,Chen, Xinhua,Li, Shuying,Li, Shuying.

[6]Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.. Li, Tian,Bi, Yu-Ping,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian. 2013

[7]Molecular cloning and expression of grass carp MyoD in yeast Pichia pastoris. Wang, Lixin,Bai, Junjie,Luo, Jianren,Chen, Hong,Ye, Xing,Jian, Qing,Lao, Haihua. 2007

[8]Porcine LEM domain-containing 3: Molecular cloning, functional characterization, and polymorphism associated with ear size. Liang Jing,Li Na,Zhang Long-chao,Wang Li-gang,Liu Xin,Zhao Ka-bin,Yan Hua,Pu Lei,Zhang Yue-bo,Shi Hui-bi,Wang Li-xian,Li Na,Zhang Qin. 2016

[9]Molecular cloning and gene expression differences of the anthocyanin biosynthesis-related genes in the red/green skin color mutant of pear (Pyrus communis L.). Yang, Ya-nan,Zhao, Guang,Zhang, Shao-ling,Gu, Chao,Wu, Jun,Yue, Wen-quan. 2013

[10]Molecular Characterization of Two Sensory Neuron Membrane Proteins From Chilo suppressalis (Lepidoptera: Pyralidae). Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Liu, Su,Qiao, Fei,Liang, Qing-Mei,Huang, Yuan-Jie,Zhou, Wen-Wu,Gong, Zhong-Jun,Cheng, Jiaan,Zhu, Zeng-Rong,Gong, Zhong-Jun. 2013

[11]Molecular Analysis of a Selenoprotein M Gene from Penaeus monodon and its Expression at Different Ovarian Stages of Development. Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui,Zhou, FaLin,Qiu, Lihua,Huang, JianHua,Yang, QiBin,Fu, Mingjun,Jiang, ShiGui. 2015

[12]Molecular cloning and characterization of a novel alpha-galactosidase gene from Penicillium sp F63 CGMCC 1669 and expression in Pichia pastoris. Mi, Shijun,Meng, Kun,Wang, Yaru,Bai, Yingguo,Yuan, Tiezheng,Luo, Huiying,Yao, Bin. 2007

[13]Characterization and molecular cloning of a serine hydroxymethyltransferase 1 (OsSHM1) in rice. Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi,Wang, Dekai,Liu, Heqin,Li, Sujuan,Zhai, Guowei,Shao, Jianfeng,Tao, Yuezhi. 2015

[14]The characterization, expression and activity analysis of superoxide dismutases (SODs) from Procambarus clarkii. Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Meng, Qingguo,Chen, Jing,Xu, Chenchao,Wang, Ya,Wang, Tingting,Zhai, Xiaoting,Gu, Wei,Wang, Wen,Huang, Yanqing. 2013

[15]Cloning, Tissue Distribution, and Transmembrane Orientation of the Olfactory Co-Receptor Orco from Two Important Lepidopteran Rice Pests, the Leaffolder (Cnaphalocrocis medinalis) and the Striped Stem Borer (Chilo suppressalis). Liu Su,Huang Yuan-jie,Qiao Fei,Zhou Wen-wu,Gong Zhong-jun,Cheng Jia-an,Zhu Zeng-rong,Gong Zhong-jun. 2013

[16]IDENTIFICATION AND CHARACTERIZATION OF TWO SENSORY NEURON MEMBRANE PROTEINS FROM Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE). Liu, Su,Zhang, Yan-Ru,Zhou, Wen-Wu,Liang, Qing-Mei,Yuan, Xin,Cheng, Jiaan,Zhu, Zeng-Rong,Gong, Zhong-Jun,Gong, Zhong-Jun. 2013

[17]Molecular cloning and characterization of hatching enzyme-like gene in the silkworm, Bombyx mori. Tang, Shun-ming,Shen, Xing-jia,Zhao, Qiao-ling,Zhang, Guo-zheng,Guo, Xi-jie,Lu, Fu-hao,Wang, Na.

[18]Molecular Cloning, Expression, and Identification of Bre Genes Involved in Glycosphingolipids Synthesis in Helicoverpa armigera (Lepidoptera: Noctuidae). Xiao, Yutao,Dhiloo, Khalid Hussain,Wu, Kongming,Soberon, Mario,Bravo, Alejandra.

[19]Molecular cloning and gene/protein expression of FAT/CD36 from grass carp (Ctenopharyngodon idella) and the regulation of its expression by dietary energy. Tian, Juan,Liu, Wei,Wu, Fan,Yu, Lijuan,Lu, Xing,Yang, Chang-Geng,Jiang, Ming,Wen, Hua,Tian, Juan,Jiang, Ming,Gao, Weihua.

[20]Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.). Fang, Rongjun,Hu, Dongqing,Zhang, Yinghua,Li, Long,Zhao, Weiguo,Liu, Li,Cheng, Jialin,Fang, Rongjun,Qi, Jinliang,Yang, Yonghua,Hu, Dongqing,Zhang, Yinghua,Zhao, Weiguo,Liu, Li.

作者其他论文 更多>>

微信小程序