Molecular detection and genome characterization of porcine circovirus type 2 in rats captured on commercial swine farms

文献类型: 外文期刊

第一作者: Zhai, Shao-Lun

作者: Zhai, Shao-Lun;Wen, Xiao-Hui;Lv, Dian-Hong;Wei, Wen-Kang;Zhai, Shao-Lun;Wen, Xiao-Hui;Lv, Dian-Hong;Wei, Wen-Kang;Zhai, Shao-Lun;Wen, Xiao-Hui;Lv, Dian-Hong;Wei, Wen-Kang;Chen, Sheng-Nan;Liu, Wei;Chen, Rui-Ai;Li, Xiao-Peng;Deng, Su-Fang;Luo, Man-Lin;Chen, Rui-Ai

作者机构:

关键词: Porcine circovirus type 2;Molecular detection;Genome characterization;Rats;Rattus norvegicus

期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Porcine circovirus type 2 (PCV2) is considered the major etiological pathogen of porcine circovirus-associated diseases (PCVADs) in pigs. Recently, PCV2 was also found in non-porcine animals such as cattle, rats, and mice. However, there was no record of PCV2 in rats in China. The goal of this study was to investigate whether PCV2 was present in rats (Rattus norvegicus, RN) on three swine farms, using molecular tools. PCR results showed that 30 of 95 (31.6 %) rat samples were positive for PCV2. Moreover, further genotype analysis suggested that 10 of 30 (33.3 %) were positive for PCV2a, 19 of 30 (63.3 %) were positive for PCV2b, and only one sample (1/30, 3.33 %) was co-infected by PCV2a and PCV2b. To determine the possible origin of PCV2, 60 serum samples were also collected from weaned pigs on those swine farms, and 23 out of 60 samples were positive for PCV2. In addition, two distinct RN-origin and two distinct porcine-origin PCV2 full-length nucleotide sequences were obtained from the farms. Sequence and phylogenetic analysis indicated that they had the highest nucleotide similarity and closest genetic relationships to each other. In this study, we report the infection and genome characterization of PCV2 in rats and compare RN-origin and porcine-origin PCV2 sequences obtained from the same pig farm, revealing possible cross-species transmission of PCV2.

分类号: R37

  • 相关文献

[1]Complete genome characterization and phylogenetic analysis of three distinct buffalo-origin PCV2 isolates from China. Zhai, Shao-Lun,He, Dong-Sheng,Qi, Wen-Bao,Deng, Su-Fang,Hu, Jun,Li, Xiao-Peng,Chen, Rui-Ai,Luo, Man-Lin,Zhai, Shao-Lun,Wei, Wen-Kang,He, Dong-Sheng,Chen, Sheng-Nan,Chen, Rui-Ai,Luo, Man-Lin,Li, Lei.

[2]First molecular detection of porcine circovirus type 2 in bovids in China. Zhai, Shao-Lun,Chen, Rui-Ai,Luo, Man-Lin,He, Dong-Sheng,Zhai, Shao-Lun,Wen, Xiao-Hui,Lv, Dian-Hong,Wu, Da-Cheng,Yuan, Jie,Huang, Zhong,Zhou, Xiu-Rong,Wei, Wen-Kang,Chen, Rui-Ai,Chen, Sheng-Nan,Luo, Man-Lin,He, Dong-Sheng.

[3]Species Abundance Distribution of Ectoparasites on Norway rats (Rattus norvegicus) from a Localized Area in Southwest China. Guo, Xian Guo,Dong, Wen Ge,Qian, Ti Jun,Wu, Dian,Ren, Tian Guang,Qin, Feng,Song, Wen Yu,Yang, Zhi Hua,Men, Xing Yuan,Fletcher, Quinn E.. 2016

[4]Out of Southern East Asia of the Brown Rat Revealed by Large-Scale Genome Sequencing. Li, Yan,Otecko, Newton O.,Liu, He-Qun,Wang, Ming-Shan,Irwin, David M.,Peng, Ming-Sheng,Wu, Dong-Dong,Zhang, Ya-Ping,Zeng, Lin,Su, Ling-Yan,Otecko, Newton O.,Liu, He-Qun,Wang, Ming-Shan,Peng, Ming-Sheng,Wu, Dong-Dong,Zhang, Ya-Ping,Ming, Chen,Li, Hai-Peng,Ming, Chen,Li, Yan,Zhang, Ya-Ping,Li, Yan,Yao, Yong-Gang,Su, Yan-Hua,Otecko, Newton O.,Peng, Ming-Sheng,Wu, Dong-Dong,Dalecky, Ambroise,Dalecky, Ambroise,Aplin, Ken,Donnellan, Stephen,Aplin, Ken,Liu, Xiao-Hui,Song, Ying,Zhang, Zhi-Bin,Esmailizadeh, Ali,Sohrabi, Saeed S.,Nanaei, Hojjat Asadollahpour,Atteynine, Solimane Ag,Atteynine, Solimane Ag,Rocamora, Gerard,Brescia, Fabrice,Morand, Serge,Irwin, David M.. 2018

[5]Seasonal variation in population characteristics and management implications for brown rats (Rattus norvegicus) within their native range in Harbin, China. Wang, Da-Wei,Yue, Ling-Fen,Huang, Bao-Huan,Li, Ning,Liu, Xiao-Hui,Cong, Lin,Wang, Yu,Zhang, Jian-Xu.

[6]Recent Evolution in Rattus norvegicus Is Shaped by Declining Effective Population Size. Deinum, Eva E.,Halligan, Daniel L.,Ness, Rob W.,Keightley, Peter D.,Zhang, Yao-Hua,Zhang, Jian-Xu,Cong, Lin.

[7]Development of a loop-mediated isothermal amplification assay for rapid detection of iridovirus in the Chinese giant salamander. Meng, Yan,Zhang, Hui,Liang, Hongwei,Zeng, Lingbing,Xiao, Hanbing,Meng, Yan,Xie, Congxin. 2013

[8]Analysis of fig tree virus type and distribution in China. Lu Mei-guang,Li Shi-fang,Zhang Zhi-xiang,He Zhen,Hong Jian,Mijit, Mahmut .. 2017

[9]Molecular detection and phylogenetic analysis of viruses causing mosaic symptoms in new sugarcane varieties in China. Wang, Xiao-Yan,Li, Wen-Feng,Huang, Ying-Kun,Zhang, Rong-Yue,Shan, Hong-Li,Yin, Jiong,Luo, Zhi-Ming.

[10]Molecular identification of Theileria parasites of northwestern Chinese Cervidae. Li, Youquan,Chen, Ze,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Li, Yaqiong,Cen, Shuangqing,Guan, Guiquan,Ren, Qiaoyun,Luo, Jianxun,Yin, Hong,Li, Youquan,Chen, Ze,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Li, Yaqiong,Cen, Shuangqing,Guan, Guiquan,Ren, Qiaoyun,Luo, Jianxun,Yin, Hong,Li, Youquan,Chen, Ze,Liu, Zhijie,Liu, Junlong,Yang, Jifei,Li, Qian,Li, Yaqiong,Cen, Shuangqing,Guan, Guiquan,Ren, Qiaoyun,Luo, Jianxun,Yin, Hong. 2014

[11]Development of a loop-mediated isothermal amplification assay combined with a lateral flow dipstick for rapid and simple detection of classical swine fever virus in the field. Chowdry, Vinay Kumar,Widen, Frederik,Belak, Sandor,Liu, Lihong,Luo, Yuzi,Qiu, Hua-Ji,Shan, Hu. 2014

[12]Development of a SCAR marker for molecular detection and diagnosis of Tilletia controversa Kuhn, the causal fungus of wheat dwarf bunt. Yu, Huixin,Gao, Fei,Liu, Taiguo,Liu, Bo,Chen, Wanquan,Yu, Huixin,Kang, Xiaohui,Han, Wensu,Gao, Fei,Gao, Jiguo.

[13]Comparative genomics provide a rapid detection of Fusarium oxysporum f. sp conglutinans. Ling Jian,Zhang Ji-xiang,Zeng Feng,Cao Yue-xia,Xie Bing-yan,Yang Yu-hong. 2016

[14]Development of a specific PCR assay for the rapid and sensitive detection of Phytophthora capsici. Lan, C. Z.,Liu, P. Q.,Li, B. J.,Chen, Q. H.,Weng, Q. Y.. 2013

[15]Loop-mediated isothermal amplification assay for sensitive and rapid detection of Phytophthora capsici. Dong, Zhongmei,Liu, Peiqing,Li, Benjin,Chen, Guoliang,Weng, Qiyong,Chen, Qinghe,Dong, Zhongmei,Chen, Guoliang,Weng, Qiyong,Chen, Qinghe.

[16]Identification of field resistance and molecular detection of the brown rust resistance gene Bru1 in new elite sugarcane varieties in China. Li, Wen-Feng,Shan, Hong-Li,Zhang, Rong-Yue,Wang, Xiao-Yan,Cang, Xiao-Yan,Yin, Jiong,Luo, Zhi-Ming,Huang, Ying-Kun,Pu, Hua-Chun. 2018

[17]Development and evaluation of specific PCR and LAMP assays for the rapid detection of Phytophthora melonis. Chen, Qinghe,Li, Benjin,Liu, Peiqing,Lan, Chengzhong,Zhan, Zhixiong,Weng, Qiyong.

[18]Protective effects of puerarin on experimental chronic lead nephrotoxicity in immature female rats. Wang, Lin,Yang, Dubao,Wang, Zhenyong,Lin, Shuqian,Li, Zifa. 2013

[19]Acute and subchronic toxicity of arprinocid in Sprague-Dawley rats. Wu, Hao,Zhang, Chong,Yan, Ming,Jiang, Shanxiang,Wu, Hao,Xiao, Wenlong,Zhang, Keyu,Xue, Feiqun,Wang, Xiaoyang. 2014

[20]Dietary Propolis Ameliorates Dextran Sulfate Sodium-Induced Colitis and Modulates the Gut Microbiota in Rats Fed aWestern Diet. Wang, Kai,Tian, Wenli,Wu, Liming,Wang, Kai,You, Mengmeng,Hu, Fuliang,Wang, Kai,Topping, David L.,Conlon, Michael A.,Jin, Xiaolu,Le Leu, Richard K.. 2017

作者其他论文 更多>>