Effect of the nucleotides surrounding the start codon on the translation of foot-and-mouth disease virus RNA

文献类型: 外文期刊

第一作者: Ma, X. X.

作者: Ma, X. X.;Feng, Y. P.;Ma, Z. R.;Gu, Y. X.;Ma, X. X.;Zhou, J. H.

作者机构:

关键词: foot-and-mouth disease virus;nucleotide bias;translation initiation;dicistronic reporter;internal ribosome entry site

期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )

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收录情况: SCI

摘要: As for the alternative AUGs in foot-and-mouth disease virus (FMDV), nucleotide bias of the context flanking the AUG2nd could be used as a strong signal to initiate translation. To determine the role of the specific nucleotide context, dicistronic reporter constructs were engineered to contain different versions of nucleotide context linking between internal ribosome entry site (IRES) and downstream gene. The results indicate that under FMDV IRES-dependent mechanism, the nucleotide contexts flanking start codon can influence the translation initiation efficiencies. The most optimal sequences for both start codons have proved to be UUU AUG1st AAC and AAG AUG2nd GAA.

分类号: R37

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