Rapid and sensitive detection of Lily symptomless virus by reverse transcription loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： He, Xiangfeng

作者： He, Xiangfeng;Wang, Wenhe;He, Xiangfeng;Wang, Wenhe;Xue, Fei;Xu, Shufa

作者机构：

关键词： Lilium spp.;RT-LAMP;LSV;Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lily symptomless virus (LSV) is one of the most prevalent viruses that infect lily plants worldwide. A rapid and sensitive reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of LSV, using two primer pairs that specifically amplified the conserved sequence of LSV coat protein. The optimum reaction conditions were as follows: 4 mM MgCl2 and 0.8 M betaine with incubation at 64 degrees C for 30 min. The limit of detection of LSV from infected lily leaves was 10-fold higher for RT-LAMP than for conventional RT-PCR. Moreover, RT-LAMP detected LSV in not only symptomatic, but also in symptomless tissues of infected plants. These findings indicate that our RT-LAMP method for LSV can serve as a low-cost, simple, and rapid alternative to conventional detection assays. (C) 2016 Elsevier B.V. All rights reserved.

分类号： R37

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