Identification of glutathione S-transferase genes in Leptinotarsa decemlineata and their expression patterns under stress of three insecticides

文献类型： 外文期刊

第一作者： Han, Jin-Bo

作者： Han, Jin-Bo;Li, Guo-Qing;Zhu, Tao-Tao;Meng, Qing-Wei;Wan, Pin-Jun

作者机构：

关键词： Glutathione S-transferase;Detoxification;Cyhalothrin;Fipronil;Endosulfan

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Glutathione S-transferases (GSTs) is a family of multifunctional enzymes that are involved in detoxification of poisonous compounds. In the present paper, the Leptinotarsa decemlineata genome and transcriptome dataset were mined and 30 GST genes were identified. These GSTs belonged to cytosolic (29 genes) and microsomal (1 gene) classes. Among them 3 GSTs (LdGSTe2, LdGSTs4, and LdGSTo3) possessed splice variants. Of the 29 cytosolic LdGSTs, 3, 10, 5, 4, 4, and I members were classified as delta, epsilon, omega, sigma, theta, and zeta subclasses respectively, along with 2 unclassified genes. Phylogenetic analysis suggest that epsilon, omega and sigma subclasses appear to undergo species -specific bloom. Moreover, most epsilon, omega and sigma GSTs are tandemly arranged in three chromosome scaffolds. To find GST candidates involving in insecticide detoxification, we tested the mRNA levels of 20 GST transcripts under stress of cyhalothrin, fipronil or endosulfan. Out of them, LdGSTe2a, LdGSTe2b, LdGSTo5 and LdGSTtI were significantly overexpressed after exposure to each of the three insecticides. Two other genes were respectively upregulated after cyhalothrin (LdGSTe10 and LdGSTu2) or endosulfan (LdGSTd1 and LdGSTu2) treatment. The diversified expression responses to insecticide exposure suggest that the LdGSTs may depend on a functionally complex system to detoxify different classes of insecticides. In addition, our findings provide a base for a better understanding of the evolution of insecticide resistance, and functional research on specific GST genes. (C) 2016 Elsevier Inc. All rights reserved.

分类号： S4

  • 相关文献

[1]Identification of carboxylesterase genes and their expression profiles in the Colorado potato beetle Leptinotarsa decemlineata treated with fipronil and cyhalothrin. Lu, Feng-Gong,Fu, Kai-Yun,Li, Qian,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun.

[2]Efficacy of endosulfan and fipronil and joint toxic action of endosulfan mixtures against Leptinotarsa decemlineata (Say). Shi, Xiao-qin,Xiong, Man-Hui,Jiang, Wei-Hua,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun. 2012

[3]Biodegradation and detoxification of endosulfan in aqueous medium and soil by Achromobacter xylosoxidans strain CS5. Yan, Yanchun,Li, Wen,Peng, Xiang,Li, Wen,Peng, Xiang,Dai, Yun,Xue, Beibei,Li, Yingying,Zhang, Jingshun.

[4]Pertussis toxin modulation of sodium channels in the central neurons of cyhalothrin-resistant and cyhalothrin-susceptible cotton bollworm, Helicoverpa armigera. Kong, De-Ling,He, Bing-Jun,Liu, Yan-Qiang,Fan, Xian-Lin,Liu, An-Xi.

[5]INFLUENCE OF PH, IONIC STRENGTH AND TEMPERATURE ON ADSORPTION OF ENDOSULFAN TO THE AQUACULTURE POND SEDIMENTS. Lin, Qin.

[6]Photocatalytic degradation of endosulfan in contaminated soil with the elution of surfactants. Xiong, Bailian,Zhang, Jinzhong,Xu, Weihong,Xiong, Bailian,Zhou, Anhong,Zheng, Guocan,Zhang, Jinzhong. 2015

[7]Adsorption and desorption characteristics of endosulfan in two typical agricultural soils in Southwest China. Qian, Sheng,Zhu, Heng,Xiong, Bailian,Zhang, Jinzhong,Xu, Weihong,Xiong, Bailian,Zheng, Guocan,Zhang, Jinzhong.

[8]Microbial degradation of endosulfan in contaminated soil with the elution of surfactants. Deng, Fei,Xiong, Bailian,Zhang, Jinzhong,Xiong, Bailian,Chen, Benshou,Zheng, Guocan,Zhang, Jinzhong.

[9]Lethal and Sublethal Effects of Endosulfan on Apolygus lucorum (Hemiptera: Miridae). Lu, Yanhui,Wu, Kongming,Liu, Yangqing,Xue, Fangsen,Wyckhuys, Kris A. G..

[10]Differential fipronil susceptibility and metabolism in two rice stem borers from China. Ye, Gong-Yin,Yao, Hong-Wei,Cheng, Jia-An,Akhtar, Zunnu-Raen,Huang, Cheng-Hua.

[11]Differential resistance and cross-resistance to three phenylpyrazole insecticides in the planthopper nilaparvata lugens (Hemiptera: Delphacidae). Zhao, Xinghua,Ning, Zuoping,Shen, Jinliang,Su, Jianya,Gao, Congfen,Zhao, Xinghua,Ning, Zuoping,Shen, Jinliang,Su, Jianya,Gao, Congfen,Zhao, Xinghua,He, Yueping.

[12]Development and validation of an ultra performance liquid chromatography Q-Exactive Orbitrap mass spectrometry for the determination of fipronil and its metabolites in tea and chrysanthemum. Liu, Xin,Lu, Chengyin. 2018

[13]Controlled Release Study on Microencapsulated Mixture of Fipronil and Chlorpyrifos for the Management of White Grubs (Holotrichia parallela) in Peanuts (Arachis hypogaea L.). Yang, Daibin,Yan, Xiaojing,Yuan, Huizhu,Li, Guangxing. 2014

[14]TOXICITIES OF FIPRONIL ENANTIOMERS TO THE HONEYBEE APIS MELLIFERA L. AND ENANTIOMERIC COMPOSITIONS OF FIPRONIL IN HONEY PLANT FLOWERS. Bao, Chen,Tao, Shu,Yang, Daibin,Zheng, Mingqi,Li, Xuefeng.

[15]Oxidative injury is involved in fipronil-induced G2/M phase arrest and apoptosis in Spodoptera frugiperda (Sf9) cell line. Wang, Xin-quan,Zhang, Hu,Wang, Xiang-yun,Qi, Pei-pei,Xu, Hao,Li, You-gui,Zhong, Shi. 2013

[16]Susceptibility to neonicotinoids and risk of resistance development in the brown planthopper, Nilaparvata lugens (St(a)over-circlel) (Homoptera: Delphacidae). Zhu, Yu Cheng,Wang, Yanhua,Chen, Jin,Ma, Chongyong,Huang, Yue,Shen, Jinliang,Wang, Yanhua. 2008

[17]Degradation of Chlorpyrifos and Fipronil in Rice from Farm to Fork and Risk Assessment. Zhang Cun-zheng,Liu Xian-jin,Tian Zi-hua,He Dan-jun,Zhang Xin-ming. 2010

[18]Fipronil resistance in the whitebacked planthopper (Sogatella furcifera): possible resistance mechanisms and cross-resistance. Li, Jian,Shao, Ying,Liu, Zewen,Tang, Jian,Yang, Baojun.

[19]Rapid and sensitive detection of fipronil and its metabolites in edible oils by solid-phase extraction based on humic acid bonded silica combined with gas chromatography with electron capture detection. Peng, Xi-Tian,Xia, Hong,Peng, Li-Jun,Li, Yu-Nan,Feng, Yu-Qi.

[20]Determination and Dissipation of Fipronil and Its Metabolites in Peanut and Soil. Li, Minghui,Li, Puyu,Wang, Lin,Feng, Mengyuan,Han, Lijun,Li, Minghui,Wang, Lin.

作者其他论文 更多>>

微信小程序