PPAR gamma is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation

文献类型： 外文期刊

第一作者： Song, Chunlei

作者： Song, Chunlei;Liang, Xiaojuan;Tao, Cong;Li, Kui;Wang, Yanfang;Yao, Jing;Cao, Chunwei;Huang, Jiaojiao;Han, Ziqiang;Zhang, Yang;Qin, Guosong;Zhao, Jianguo;Yao, Jing;Cao, Chunwei;Huang, Jiaojiao;Zhang, Yang;Qin, Guosong;Zhao, Jianguo;Han, Ziqiang;Li, Chengbo;Yang, Haoran

作者机构：

关键词： Pig;miRNA;Oocyte maturation;PPAR gamma;Fatty acid metabolism

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To elucidate the key miRNAs and the signalling pathways that are involved in porcine oocyte maturation, we performed a deep sequencing analysis of the miRNAs of pig germinal vesicle (GV) oocytes and metaphase II (MII) oocytes. Seven differentially expressed (DE) miRNAs were identified and the expression levels of miR-21 and miR-27b-3p were further confirmed by QPCR analysis. The target genes of 7 DE miRNAs were predicted and subjected to pathway analysis. Interestingly, fatty acid metabolism and fatty acid biosynthesis were the top two significantly enriched molecular functions during oocyte maturation. Heat map, which was built with 7 DE miRNAs and the enriched the molecular functions, revealed that miR-21, miR-27b-3p, miR-10a-5p and miR-10b-5p were involved in fatty acid metabolism. In particular, the regulatory role of miR-27b-3p on peroxisome proliferator-activated receptor-gamma (PPAR gamma) was confirmed by their inversed expression patterns in GV and MII oocytes and luciferase report assays. In addition, we observed that PPAR gamma agonist (rosiglitazone) treatment significantly enhanced porcine oocyte maturation rate and early embryo developmental competent. Taken together, our results demonstrated that miR-27b and its target, PPAR gamma, play the vital roles in pig oocyte maturation through regulating the fatty acid metabolism. These data increased our understanding of the regulatory gene networks in porcine oocyte maturation and development. (C) 2016 Elsevier Inc. All rights reserved.

分类号： Q5

  • 相关文献

[1]miRNAome, mRNAome and degradome analysis of Tibetan minipigs anterior pituitary. Ye, Rui-Song,Li, Meng,Chen, Ting,Wei, Xiao-Chen,Qi, Qi-En,Cheng, Xiao,Li, Chao-Yun,Jiang, Qing-Yan,Xi, Qian-Yun,Zhang, Yong-Liang. 2018

[2]Repertoire of porcine MicroRNAs in adult ovary and testis by deep sequencing. Chen, Haosi,Wang, Xin,Gao, Xiaolian,Li, Mingzhou,Liu, Yingkai,Wang, Tao,Guan, Jiuqiang,Luo, Zonggang,Ma, Jideng,Mu, Zhiping,Jiang, An-an,Zhu, Li,Li, Xuewei,Chen, Lei,Wang, Jinyong,Lang, Qiulei,Zhou, Xiaochuan,Zeng, Wenxian,Li, Ning,Li, Kui.

[3]The microRNA expression profile in porcine skeletal muscle is changed by constant heat stress. Hao, Y.,Liu, J. R.,Yang, P. G.,Feng, Y. J.,Cui, Y. J.,Yang, C. H.,Gu, X. H.,Liu, J. R.,Zhang, Y..

[4]Dual role of IGF-II in oocyte maturation in southern flounder Paralichthys lethostigma: Up-regulation of mPR alpha and resumption of meiosis. Picha, Matthew E.,Shi, Bao,Thomas, Peter,Shi, Bao,Shi, Bao. 2012

[5]Role of animal pole protuberance and microtubules during meiosis in sea cucumber Apostichopus japonicus oocytes. Chang Yaqing,Yu Jiaping,Sun Huiling,Pang Zhenguo,Pang Zhenguo. 2010

[6]IGF-I slightly improves nuclear maturation and cleavage rate of bovine oocytes exposed to acute heat shock in vitro. Qi Meiyu,Liu, Di.

[7]Characterization of two Delta(5) fatty acyl desaturases in abalone (Haliotis discus hannai Ino). Li, Mingzhu,Mai, Kangsen,He, Gen,Ai, Qinghui,Zhang, Wenbing,Xu, Wei,Liufu, Zhiguo,Zhang, Yanjiao,Zhou, Huihui,Li, Mingzhu,Mai, Kangsen,He, Gen,Ai, Qinghui,Zhang, Wenbing,Xu, Wei,Liufu, Zhiguo,Zhang, Yanjiao,Zhou, Huihui,Wang, Jia.

[8]Evolution of the Aroma Volatiles of Pear Fruits Supplemented with Fatty Acid Metabolic Precursors. Qin, Gaihua,Tao, Shutian,Zhang, Huping,Huang, Wenjiang,Wu, Juyou,Zhang, Shaoling,Qin, Gaihua,Qin, Gaihua,Xu, Yiliu.

[9]Construction and characterization of a BIBAC library of Jatropha curcas L. and identification of BIBAC clones containing genes associated with fatty acid metabolism. Fu, Yuhua,Wang, Haiyan,Wang, Wenquan,Scheuring, Chantel F.,Zhang, Hong-bin,Fu, Yuhua. 2011

[10]Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation. Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Zang, Kun,Ling, Zongshuai. 2012

[11]Isoflavones in Chickpeas Inhibit Adipocyte Differentiation and Prevent Insulin Resistance in 3T3-L1 Cells. Yao, Yang,Ren, Guixing,Gao, Yue,Zhu, Yinging.

[12]Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation. Zhu, Yingying,Yao, Yang,Gao, Yue,Hu, Yibo,Shi, Zhenxing,Ren, Guixing,Zhu, Yingying,Gao, Yue.

[13]Anti-adipogenic Effects of Polyphenol Extracts of Areca Flower Tea on 3T3-L1 Preadipocytes. Song, Fei,Tang, MinMin,Shen, XiaoJun,Wang, Hui,Chen, Hua,Zhao, SongLin,Wu, QiuSheng.

[14]Troglitazone Induced Apoptosis via PPAR gamma Activated POX-induced ROS Formation in HT29 Cells. Wang Jing,Du YuGuo,Lv XiaoWen,Shi JiePing,Hu XiaoSong. 2011

[15]Postnatal expression pattern of PPAR gamma gene in pigs. Jiang, Junfang,Jiang, Yongqing,Zhu, Zhigang,Xu, Zirong. 2009

[16]Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells. Wang, Jing,Lv, Xiao-Wen,Shi, Jie-Ping,Hu, Xiao-Song. 2007

[17]A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor gamma with miR-27 involved mechanism. Zhu, Wei,Zou, Bo,Nie, Rongzu,Zhang, Ying,Li, Chun-mei,Zou, Bo,Li, Chun-mei.

[18]Identification and characterization of transcript variants of chicken peroxisome proliferator-activated receptor gamma. Duan, Kui,Sun, Yingning,Zhang, Xiaofei,Zhang, Wenjian,Zhang, Jiyang,Wang, Guihua,Wang, Shouzhi,Leng, Li,Li, Hui,Wang, Ning,Duan, Kui,Sun, Yingning,Zhang, Xiaofei,Zhang, Tianmu,Zhang, Wenjian,Zhang, Jiyang,Wang, Guihua,Wang, Shouzhi,Leng, Li,Li, Hui,Wang, Ning,Sun, Yingning.

[19]Peanut (Arachis hypogaea L.) Omics and Biotechnology in China. Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Liu, Shuan-Tao. 2011

[20]Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development. Gao, Chao,Wang, Pengfei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Wang, Xingjun,Ju, Zheng. 2017

作者其他论文 更多>>

微信小程序