Isoquercitrin production from rutin catalyzed by naringinase under ultrasound irradiation

文献类型： 外文期刊

第一作者： Zhu, Dan

作者： Zhu, Dan;Gong, An;Xu, Yan;Tsabing, D'assise Kinfack;Wu, Fuan;Wang, Jun;Wu, Fuan;Wang, Jun

作者机构：

关键词： Ultrasound irradiation;Enzymatic hydrolysis;Isoquercitrin;Naringinase;Surface plasmon resonance

期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）

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年卷期：

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收录情况： SCI

摘要： Isoquercitrin, a rare flavonol glycoside with wide biological activities and key synthetic intermediate for the production of enzymatically modified isoquercitrin (EMIQ), was conducted by naringinase-catalyzed conversion of rutin under ultrasound irradiation. The maximum yields were obtained to 98.35 3.13% and 95.20 +/- 2.52% under conventional heating and ultrasound irradiation, respectively. The optimal results under ultrasound irradiation were obtained under the following conditions: rutin concentration 0.8 naringinase concentration 3000 U/L, reaction temperature 40 degrees C for 20 min, which was more economical than that with conventional heating. The reaction time was reduced from 60 min to 20 min, and the apparent kinetic parameter (V-m/K-m) was increased 3.72-fold. The lower activity energy Ea under ultrasonic irradiation was 0.7-fold of that in an incubator reactor, which could easily initiate the enzymatic reaction. The association saturation constant K-a was 1.98-fold higher than that with conventional heating, showed a better affinity between rutin and naringinase detected by surface plasmon resonance (SPR) analysis. These results suggest that ultrasound irradiation can accelerate the enzymatic synthesis of isoquercitrin from rutin. (C) 2016 Elsevier B.V. All rights reserved.

分类号： O64`Q55`O643

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