Isolation of four rice seed-specific promoters and evaluation of endosperm activity

文献类型： 外文期刊

第一作者： Xu, Rongfang

作者： Xu, Rongfang;Li, Dongdong;Yang, Yachun;Li, Li;Yang, Jianbo;Xu, Rongfang;Li, Dongdong;Li, Hao;Li, Juan;Yang, Yachun;Qin, Ruiying;Li, Li;Wei, Pengcheng;Yang, Jianbo

作者机构：

关键词： Endosperm specific;Promoter;Rice;Transgene;Biotechnology

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Cereal grains are major targets for genetically improving the nutritional value of food and for producing recombinant proteins. Strong and tissue-specific promoters are highly desired for effectively controlling expression in the seed or endosperm. In this study, we isolated four rice promoters from the 5' upstream region of putative seed-specifically expressed genes: PROLAM26, RAL2, RAL4 and CAPIP. By generating transgenic rice plants carrying promoter-reporter constructs, we found these four promoters to be specifically expressed in seeds, with three having endosperm-specific or -preferential activity. The strength of each promoter in the endosperm was determined and compared to a constitutively expressed OsACTIN promoter and an endosperm-specifically expressed Glu4-B promoter in single-copy transgenic plants. The promoter of RAL2 exhibited relatively high activity, and the promoters of RAL4 and CAPIP exhibited activities comparable with those of OsACTIN and Glu4-B. In addition, monitoring activities in high-generation (T-3-T-4) homozygous progeny of single-copy plants revealed maintenance of expression for all four promoters, with no evidence of silencing. Taken together, our findings offer four stable rice seed-specific promoters of different strengths for endosperm expression.

分类号： Q942

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