Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco

文献类型： 外文期刊

第一作者： Li, Tian

作者： Li, Tian;Sun, Jingkuan;Bi, Yuping;Peng, Zhenying

作者机构：

关键词： MYB transcription factor;Fraxinus velutina;Salt tolerance;Transgenic tobacco

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MYB transcription factors (TFs) in plants play important roles in abiotic stress tolerance. Here, we report that an MYB-related TF gene, named FvMYB1, was isolated from the salt-tolerant tree species Fraxinus velutina Torr. The real-time PCR analysis showed that the FvMYB1 transcript increased remarkably in response to salt treatment. Subsequently, the FvMYB1 gene was transferred into the tobacco genome via the Agrobacterium-mediated approach. PCR and Southern blot verified that the FvMYB1 gene had been expressed in transgenic tobacco. The over-expressing transgenic seedlings displayed better salt tolerance in root length, shoot fresh weight, and shoot/root ratio compared to the wild type. Moreover, along with the superoxide dismutase and peroxidase activities and proline content that were significantly increased in the transgenic plants, the transcriptions of a series of stress-responsive genes were up-regulated under salt treatment. Further, an analysis of the upstream sequence of the FvMYB1 gene revealed that the promoter could enhance GUS gene expression in transgenic tobacco with NaCl treatment. These results indicate that FvMYB1 functions as a transcriptional activator that plays a regulatory role in salt stress tolerance of F. velutina.

分类号： Q94

  • 相关文献

[1]Expression of a wheat MYB gene in transgenic tobacco enhances resistance to Ralstonia solanacearum, and to drought and salt stresses. Liu, Hongxia,Zhou, Xianyao,Dong, Na,Liu, Xin,Zhang, Zengyan,Zhou, Xianyao,Zhang, Huaiyu.

[2]Isolation and functional characterisation of CDPKs gene from Arachis hypogaea under salt stress. Li, Yan,Guo, Feng,Meng, Jing-Jing,Li, Xin-Guo,Li, Yan,Wan, Shu-Bo,Fang, Feng,Xia, Guang-Min.

[3]A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco. Li, Tian,Sun, Jingkuan,Li, Tian,Bi, Yuping. 2017

[4]Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping. Wang, Fengqing,Suo, Yanfei,Chen, Xinjian,Zhang, Zhongyi,Wei, He,Li, Mingjie,Zhang, Zhongyi,Xie, Caixia,Wang, Lina. 2015

[5]Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress. Zhang, Lichao,Zhao, Guangyao,Jia, Jizeng,Liu, Xu,Kong, Xiuying.

[6]Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease. Liu, Xin,Yang, Lihua,Zhou, Xianyao,Lu, Yan,Zhang, Zengyan,Yang, Lihua,Ma, Lingjian,Zhou, Miaoping,Ma, Hongxiang.

[7]GaMYB85, an R2R3 MYB gene, in transgenic Arabidopsis plays an important role in drought tolerance. Butt, Hamama Islam,Li, Fuguang,Zhang, Xueyan,Yang, Zhaoen,Gong, Qian,Chen, Eryong,Wang, Xioaqian,Zhao, Ge,Ge, Xiaoyang,Zhang, Xueyan,Li, Fuguang. 2017

[8]FtSAD2 and FtJAZ1 regulate activity of the FtMYB11 transcription repressor of the phenylpropanoid pathway in Fagopyrum tataricum. Zhou, Meiliang,Sun, Zhanmin,Ding, Mengqi,Wang, Dan,Tang, Yixiong,Wu, Yanmin,Zhou, Meiliang,Zhu, Xuemei,Logacheva, Maria D.,Kreft, Ivan,Yan, Mingli,Shao, Jirong. 2017

[9]Soybean transcription factor GmMYBZ2 represses catharanthine biosynthesis in hairy roots of Catharanthus roseus. Zhou, Mei-Liang,Shao, Ji-Rong,Zhou, Mei-Liang,Wu, Yan-Min,Tang, Yi-Xiong,Hou, Hong-Li,Zhu, Xue-Mei.

[10]Changing a conserved amino acid in R2R3-MYB transcription repressors results in cytoplasmic accumulation and abolishes their repressive activity in Arabidopsis. Zhou, Meiliang,Sun, Zhanmin,Wang, Chenglong,Tang, Yixiong,Wu, Yanmin,Wang, Chenglong,Shao, Jirong,Zhang, Xinquan,Zhu, Xuemei.

[11]BjMYB1, a transcription factor implicated in plant defence through activating BjCHI1 chitinase expression by binding to a W-box-like element. Gao, Ying,Jia, Shuangwei,Wang, Chunlian,Wang, Fujun,Wang, Fajun,Zhao, Kaijun.

[12]OsARM1, an R2R3 MYB Transcription Factor, Is Involved in Regulation of the Response to Arsenic Stress in Rice. Wang, Feng-Zhu,Yu, Lu-Jun,Xie, Li-Juan,Yuan, Li-Bing,Qi, Hua,Xiao, Ming,Guo, Wuxiu,Shu, Wensheng,Xiao, Shi,Chen, Qin-Fang,Chen, Mo-Xian,Zhang, Jianhua,Chen, Zhe,Qiu, Rongliang,Yi, Keke,Zhang, Jianhua,Zhang, Jianhua. 2017

[13]An R2R3 MYB transcription factor in wheat, TaPIMP1, mediates host resistance to Bipolaris sorokiniana and drought stresses through regulation of defense- and stress-related genes. Liu, Xin,Wang, Xindong,Zhou, Xianyao,Ye, Xingguo,Wei, Xuening,Zhou, Miaoping. 2012

[14]Molecular cloning and expression of a novel MYB transcription factor gene in rubber tree. Qin, Bi,Zhang, Yu,Wang, Meng.

[15]Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis. Chen, SC,Peng, SQ,Huang, GX,Wu, KX,Fu, XH,Chen, ZQ.

[16]HbMyb1, a Myb transcription factor from Hevea brasiliensis, suppresses stress induced cell death in transgenic tobacco. Peng, Shi-Qing,Wu, Kun-Xin,Chen, Shou-Cai,Chen, Shou-Cai,Huang, Gui-Xiu.

[17]Overexpression of CaHSP26 in transgenic tobacco alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance. Guo, Shang-Jing,Zhou, Hai-Yan,Zhang, Xian-Sheng,Li, Xin-Guo,Meng, Qing-Wei. 2007

[18]Overexpression of a tomato flavanone 3-hydroxylase-like protein gene improves chilling tolerance in tobacco. Zhang, Song,Wang, Guo-Dong,Kong, Fan-Ying,Meng, Chen,Deng, Yong-Sheng.

[19]Identification and differential expression of two isogenes encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Glycine max. Zhang, Man,Li, Kai,Liu, Jianyu,Yu, Deyue,Zhang, Man. 2012

[20]A Cu/Zn superoxide dismutase gene from Saussurea involucrata Kar. & Kir., SiCSD, enhances drought, cold, and oxidative stress in transgenic tobacco. Zhang, L.,Sun, L.,Zhang, L.,Qiu, H.,Liu, C.,Wang, A.,Zhu, J.,Deng, F.. 2017

作者其他论文 更多>>

微信小程序