Effects of Calcium and Calmodulin Antagonists on Chilling Stress-Induced Proline Accumulation in Jatropha curcas L.

文献类型： 外文期刊

第一作者： Yang, Shuang-Long

作者： Yang, Shuang-Long;Deng, Feng-Fei;Gong, Ming;Lan, Shan-Shan

作者机构：

关键词： Calcium;Calmodulin antagonists;Proline biosynthesis and degradation;Chilling stress;Chilling tolerance;Jatropha curcas

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Regulation of proline accumulation in plants under chilling stress remains unclear. In this paper, we treated Jatropha curcas seedlings under chilling stress with exogenous calcium chloride (CaCl2), the plasma membrane Ca2+-channel blocker lanthanum chloride (LaCl3), calmodulin antagonists, chlorpromazine (CPZ), and trifluoperazine (TFP) and investigated the effects of calcium and calmodulin (CaM) on proline accumulation and chilling tolerance. The results showed that CaCl2 treatment significantly enhanced chilling stress-induced proline accumulation. CaCl2 also induced an almost immediate and rapid increase of Delta 1-pyrroline-5-carboxylate synthetase (P5CS) and glutamate dehydrogenase activities, the key enzymes in the glutamate pathway of proline biosynthesis, and up-regulated P5CS expression, but it decreased the activity of proline dehydrogenase (ProDH), a key enzyme of proline degradation, and inhibited ProDH expression. Treatment with LaCl3, CPZ, and TFP exhibited the opposite effects to those by CaCl2 treatment. Moreover, CaCl2, LaCl3, CPZ, and TFP had little effect on the activities of ornithine aminotransferase and arginase, the key enzymes in the ornithine pathway of proline biosynthesis. These results indicated that Ca2+-CaM might be involved in signal transduction events, leading to proline accumulation in J. curcas seedlings under chilling stress, and that Ca2+-induced proline accumulation is a combined result of the activation of the glutamate pathways of proline biosynthesis and the simultaneous inhibition of the proline degradation pathway. In addition, CaCl2 treatment increased tissue vitality, decreased the content of the lipid peroxidation product malondialdehyde (MDA), and alleviated electrolyte leakage in J. curcas seedlings under chilling stress, indicating that exogenous Ca2+ can enhance chilling tolerance, and proline might be a key factor in this increased chilling tolerance.

分类号： Q94

  • 相关文献

[1]Variation of photosynthetic tolerance of rice cultivars (Oryza sativa L.) to chilling temperature in the light. Li, Xia,Cao, Kun,Wang, Chao,Sun, Zhi-wei,Yan, Lina. 2010

[2]Calcium influence on chilling resistance of grafting eggplant seedlings. Gao, HB,Chen, GL,Han, LH,Lin, HA. 2004

[3]Development of highly regenerable callus lines and Agrobacterium-mediated transformation of Chinese lawngrass (Zoysia sinica Hance) with a cold inducible transcription factor, CBF1. Li, RF,Wei, JH,Wang, HZ,He, J,Sun, ZY. 2006

[4]Genotype x environment interactions for chilling tolerance of rice recombinant inbred lines under different low temperature environments. Jiang, Wenzhu,Lee, Joohyun,Chu, Sang-Ho,Ham, Tae-Ho,Woo, Mi-Ok,Cho, Young-Il,Koh, Hee-Jong,Jiang, Wenzhu,Lee, Joohyun,Chu, Sang-Ho,Ham, Tae-Ho,Woo, Mi-Ok,Cho, Young-Il,Koh, Hee-Jong,Chin, Joong-Hyoun,Han, Longzhi,Xuan, Yingshi,Yuan, Donglin,Xu, Furong,Dai, Luyuan,Yea, Jong-Doo.

[5]Salicylic-Acid-Induced Chilling- and Oxidative-Stress Tolerance in Relation to Gibberellin Homeostasis, C-Repeat/Dehydration-Responsive Element Binding Factor Pathway, and Antioxidant Enzyme Systems in Cold-Stored Tomato Fruit. Ding, Yang,Zhao, Jinhong,Nie, Ying,Fan, Bei,Wu, Shujuan,Zhang, Yu,Tang, Xuanming,Sheng, Jiping,Shen, Lin,Zhao, Ruirui.

[6]Low-temperature conditioning induces chilling tolerance in 'Hayward' kiwifruit by enhancing antioxidant enzyme activity and regulating en-dogenous hormones levels. Yang, Qingzhen,Rao, Jingping,Wang, Yuping,Sun, Zhenying,Ma, Qiushi,Dong, Xiaoqing,Yang, Qingzhen,Zhang, Zhengke. 2013

[7]Stress-responsive gene ICE1 from Vitis amurensis increases cold tolerance in tobacco. Dong, Chang,Zhang, Zhen,Ren, Junpeng,Huang, Jinfeng,Wang, Yan,Cai, Binhua,Tao, Jianmin,Dong, Chang,Qin, Yang,Wang, Bailin. 2013

[8]Effects of Postharvest Gibberellic Acid Treatment on Chilling Tolerance in Cold-Stored Tomato (Solanum lycopersicum L.) Fruit. Zhu, Zhen,Ding, Yang,Zhao, Jinhong,Nie, Ying,Zhang, Yu,Tang, Xuanming,Sheng, Jiping.

[9]Inhibition of glutathione synthesis decreases chilling tolerance in Chorispora bungeana callus. Wu, Jianmin,Zhao, Zhiguang,An, Lizhe,Liu, Yanhong,Xu, Shijian,Gao, Dahai,An, Lizhe,Zhang, Youfu,Liu, Yanhong. 2008

[10]A P4-ATPase Gene GbPATP of Cotton Confers Chilling Tolerance in Plants. Liu, Tingli,Guo, Shiwei,Lian, Ziyi,Chen, Fei,Yang, Yuwen,Chen, Tianzi,Ling, Xitie,Liu, Aiming,Zhang, Baolong,Wang, Rongfu.

[11]Molecular characterization of a cold-responsive RING-H2 finger gene from banana fruit and its interaction with MaMYC2a. Chen, Jiao,Kuang, Jian-Fei,Shan, Wei,Wang, Jun-ning,Xiao, Yun-yi,Chen, Jian-ye,Lu, Wang-jin,Chen, Jiao,Wang, Jun-ning.

[12]Cucumber (Cucumis sativus L.) over-expressing cold-induced transcriptome regulator ICE1 exhibits changed morphological characters and enhances chilling tolerance. Liu, Liying,Duan, Liusheng,Zhang, Jiachang,Zhang, Zhenxian,Ren, Huazhong,Mi, Guoquan.

[13]Quantitative determination of phorbol ester derivatives in Chinese Jatropha curcas seeds by high-performance liquid chromatography/mass spectrometry. Li, Li,Li, Wenqing,Chen, Fang,Li, Jing,Lu, Daihua,Chen, Fang,Li, Jing. 2013

[14]Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield. Xia, Zhiqiang,Zhang, Shengkui,Wen, Mingfu,Lu, Cheng,Sun, Yufang,Zou, Meiling,Wang, Wenquan,Xia, Zhiqiang,Zhang, Shengkui,Zou, Meiling. 2018

[15]Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis. Chen, Y.,Zhou, B.,Zhou, L. R.,Cai, J.,Yang, F. X.. 2015

[16]Genetic variation and phylogenetic relationship analysis of Jatropha curcas L. inferred from nrDNA ITS sequences. Guo, Guo-Ye,Chen, Fang,Shi, Xiao-Dong,Tian, Yin-Shuai,Guo, Guo-Ye,Yu, Mao-Qun,Han, Xue-Qin,Yuan, Li-Chun,Zhang, Ying.

[17]IMPROVEMENT ON THE THERMOSTABILITY AND ACTIVITY OF APX1 FROM ENERGY PLANT JATROPHA CURCAS L. BY HYPER-ACIDIC FUSION PARTNERS. Zhang, Mengru,Yang, Yumei,Li, Xujuan,Wang, Haibo,Yang, Shuanglong,Wang, Shasha,Gong, Ming,Zou, Zhurong,Li, Xujuan,Wang, Haibo. 2014

[18]Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant. Zhang, Chao,Fu, Shaoping,Guo, Jianchun,Zhang, Chao,Zhang, Chao,Tang, Gaijuan,Hu, Xinwen. 2013

[19]Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing. 2014

[20]Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas. Wei, Qian,Li, Jun,Zhang, Lin,Wu, Pingzhi,Chen, Yaping,Li, Meiru,Jiang, Huawu,Wu, Guojiang,Wei, Qian,Zhang, Lin,Li, Jun. 2012

作者其他论文 更多>>

微信小程序