Isolation and characterisation of peroxiredoxin 6 promoter from sheep (Ovis aries)
文献类型: 外文期刊
第一作者: Liu, Nan-Nan
作者: Liu, Nan-Nan;Liu, Zeng-Shan;Lu, Shi-Ying;Hu, Pan;Zhang, Ying;Li, Yan-Song;Zhou, Yu;Ren, Hong-Lin;Fu, Bao-Quan;Zhang, Yu
作者机构:
关键词: Ovis aries;peroxiredoxin 6;promoter;transcriptional activity
期刊名称:JOURNAL OF VETERINARY RESEARCH ( 影响因子:1.744; 五年影响因子:1.838 )
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收录情况: SCI
摘要: Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein and a unique 1-Cys Prdx of the peroxiredoxin family. The expression and regulation of Prdx6 are implicated in numerous physiological and pathological processes. Material and Methods: Eight stepwise truncated DNA fragments obtained from the 5'-flank region of the Prdx6 gene were prepared and subcloned into the pSEAP2-Enhancer vectors. To investigate the transcriptional activity of the truncated DNA fragments, the recombinant plasmids were transfected into the COS-1 cells and the transcriptional activity was measured via assaying the expression of the reporter gene of the secreted alkaline phosphatase. Results: A 3.4 kb 5'-upstream flank region of the Prdx6 gene was cloned and sequenced. The region from -108 nt to -36 nt of the 5'-flanking region of the Prdx6 gene contained basal transcriptional activity. Conclusion: This result provides the basis for further studies on the gene regulation of the Prdx6-mediated biological processes and on screening for the transacting factors that interact with cis-acting elements of the Prdx6 gene promoter.
分类号: S85
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