Cooperative Reinforcement of Ionic Liquid and Reactive Solvent on Enzymatic Synthesis of Caffeic Acid Phenethyl Ester as an In Vitro Inhibitor of Plant Pathogenic Bacteria

文献类型： 外文期刊

第一作者： Xu, Yan

作者： Xu, Yan;Sheng, Sheng;Liu, Xi;Wang, Chao;Xiao, Wei;Wang, Jun;Wu, Fu-An;Xu, Yan;Sheng, Sheng;Wang, Jun;Wu, Fu-An

作者机构：

关键词： biocatalysis;ionic liquid;caffeic acid phenethyl ester;reactive solvent;Ralstonia solanacearum;antibacterial activity

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： It is widely believed that lipases in ionic liquids (ILs) possess higher enzyme activity, stability and selectivity; however, reaction equilibrium is always limited by product inhibition, and the product is difficult to separate from non-volatile ILs using distillation. To solve this problem, using trialkylphosphine oxide (TOPO) as a complexing agent, a novel biphase of reactive solvent and IL was firstly reported for caffeic acid phenethyl ester (CAPE) production from methyl caffeate (MC) and 2-phenylethanol (PE) catalyzed by lipase via transesterification. The effects of the reaction parameters and their action mechanism were investigated, and the inhibition of CAPE against bacterial wilt pathogen Ralstonia solanacearum was firstly measured. The MC conversion of 98.83% +/- 0.76% and CAPE yield of 96.29% +/- 0.07% were obtained by response surface methodology in the 25 g/L TOPO-cyclohexane/[Bmim][Tf2N] (1:1, v/v); the complex stoichiometry calculation and FTIR spectrum confirmed that the reversible hydrogen- bond complexation between TOPO and caffeates significantly enhances the cooperative effect of two phases on the lipase-catalyzed reaction. The temperature was reduced by 14 degrees C; the MC concentration increased by 3.33-fold; the ratio of catalyst to donor decreased by 4.5-fold; and Km decreased 1.08-fold. The EC50 of CAPE against R. solanacearum was 0.17-0.75 mg/mL, suggesting that CAPE is a potential in vitro inhibitor of plant pathogenic bacteria.

分类号： O62

  • 相关文献

[1]Enhancement of Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquid with DMSO Co-solvent. Gu, Shuangshuang,Wang, Jun,Wei, Xianbin,Cui, Hongsheng,Wu, Xiangyang,Wu, Fuan,Wang, Jun,Wang, Jun,Wu, Fuan. 2014

[2]Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquids: Effect of Specific Ions and Reaction Parameters. Wang Jun,Li Jing,Zhang Leixia,Gu Shuangshuang,Wu Fuan,Wu Fuan. 2013

[3]A novel continuous flow biosynthesis of caffeic acid phenethyl ester from alkyl caffeate and phenethanol in a packed bed microreactor. Wang, Jun,Gu, Shuang-Shuang,Cui, Hong-Sheng,Wang, Jun,Wu, Xiang-Yang,Wu, Fu-An.

[4]A novel chemoenzymatic synthesis of propyl caffeate using lipase-catalyzed transesterification in ionic liquid. Pang, Na,Gu, Shuang-Shuang,Wang, Jun,Cui, Hong-Sheng,Wang, Fang-Qin,Liu, Xi,Zhao, Xing-Yu,Wu, Fu-An,Wu, Fu-An.

[5]Enhancement of the selective enzymatic biotransformation of rutin to isoquercitrin using an ionic liquid as a co-solvent. Wang, Jun,Sun, Guo-Xia,Wu, Fu-An,Guo, Xi-Jie,Yu, Liang,Wu, Fu-An,Guo, Xi-Jie,Yu, Liang.

[6]Ultrasound irradiation accelerates the lipase-catalyzed synthesis of methyl caffeate in an ionic liquid. Wang, Jun,Wang, Shasha,Li, Zhongjian,Gu, Shuangshuang,Wu, Fuan,Wang, Jun,Wang, Shasha,Li, Zhongjian,Gu, Shuangshuang,Wu, Fuan,Wang, Jun,Wu, Xiangyang.

[7]New Insights into the Antibacterial Activity of Hydroxycoumarins against Ralstonia solanacearum. Yang, Liang,Ding, Wei,Wu, Dousheng,Li, Shili,Chen, Juanni,Guo, Bing,Xu, Yuquan.

[8]Antibacterial activity against Ralstonia solanacearum of phenolic constituents isolated from dragon's blood. Wang, Hui,Luo, Ying,Dai, Haofu,Mei, Wenli.

[9]Generic DART-MS platform for monitoring the on-demand continuous-flow production of pharmaceuticals: Advancing the quantitative protocol for caffeates in microfluidic biocatalysis. Xu, Yan,Zhang, Dong-Yang,Liu, Xi,Sheng, Sheng,Wu, Guo-Hua,Wang, Jun,Wu, Fu-An,Xu, Yan,Zhang, Dong-Yang,Sheng, Sheng,Wu, Guo-Hua,Wang, Jun,Wu, Fu-An,Meng, Xiang-Yun.

[10]Green synthesis of gold nanoparticles using Citrus maxima peel extract and their catalytic/antibacterial activities. Yuan, Chun-Gang,Huo, Can,Gui, Bing,Cao, Wei-Ping. 2017

[11]Enantio-selective preparation of (S)-1-phenylethanol by a novel marine GDSL lipase MT6 with reverse stereo-selectivity. Deng, Dun,Zhang, Yun,Sun, Aijun,Hu, Yunfeng,Deng, Dun,Zhang, Yun,Sun, Aijun,Hu, Yunfeng,Deng, Dun,Hu, Yunfeng. 2016

[12]Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis. Zhang, Hongfang,Su, Xiaoyun,Ang, Ee Lui,Zhao, Huimin,Li, Xiukai,Zhang, Yugen,Su, Xiaoyun,Zhao, Huimin,Zhao, Huimin,Zhao, Huimin. 2016

[13]Chemical structures, biosynthesis, bioactivities, biocatalysis and semisynthesis of tobacco cembranoids: An overview. Yan, Ning,Du, Yongmei,Liu, Xinmin,Zhang, Hongbo,Liu, Yanhua,Zhang, Peng,Gong, Daping,Zhang, Zhongfeng.

[14]Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition. Zheng, Ming-Ming,Huang, Feng-Hong,Wang, Lian,Guo, Ping-Mei,Deng, Qian-Chun,Zheng, Ming-Ming,Huang, Feng-Hong,Wang, Lian,Guo, Ping-Mei,Deng, Qian-Chun,Lu, Yong,Feng, Yu-Qi.

[15]Selective hydrolysis by commercially available hesperidinase for isoquercitrin production. Wang, Jun,Ma, Yan-Long,Xia, Rui,Sun, Guo-Xia,Wu, Fu-An,Wang, Jun,Wu, Xiang-Yang,Yu, Liang,Wu, Fu-An,Yu, Liang.

[16]Structured lipids enriched with unsaturated fatty acids produced by enzymatic acidolysis of silkworm pupae oil using oleic acid. Zhao, Xing-Yu,Wang, Xu-Dong,Liu, Xi,Zhu, Wei-Jie,Mei, Yi-Yuan,Li, Wen-Wen,Wang, Jun,Wang, Jun.

[17]Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters. Zheng, Ming-Ming,Dong, Ling,Guo, Ping-Mei,Deng, Qian-Chun,Li, Wen-Lin,Huang, Feng-Hong,Lu, Yong,Feng, Yu-Qi.

[18]From microalgae oil to produce novel structured triacylglycerols enriched with unsaturated fatty acids. Wang, Jun,Wang, Xu-Dong,Zhao, Xing-Yu,Liu, Xi,Wu, Fu-An,Wang, Jun,Wu, Fu-An,Dong, Tao.

[19]Microfluidic biocatalysis enhances the esterification of caffeic acid and methanol under continuous-flow conditions. Wang, Sha-Sha,Li, Zhong-Jian,Sheng, Sheng,Wu, Fu-An,Wang, Jun,Sheng, Sheng,Wu, Fu-An,Wang, Jun.

[20]Electricity generation from starch processing wastewater using microbial fuel cell technology. Lu, Na,Zhang, Jin-tao,Ni, Jin-ren,Lu, Na,Zhou, Shun-gui,Zhang, Jin-tao.

作者其他论文 更多>>

微信小程序