Isolation and Characterization of a Phaseolus vulgaris Trypsin Inhibitor with Antiproliferative Activity on Leukemia and Lymphoma Cells

文献类型： 外文期刊

第一作者： Li, Miao

作者： Li, Miao;Wang, Hexiang;Li, Miao;Wang, Hexiang;Li, Miao;Cui, Yajuan;Li, Dong;Liu, Qin;Ng, Tzi Bun

作者机构：

关键词： leguminous seeds;leukemia L1210;lymphoma MBL2;purification

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A 17.5-kDa trypsin inhibitor was purified from Phaseolus vulgaris cv. gold bean with an isolation protocol including ion exchange chromatography on DEAE-cellulose (Diethylaminoethyl-cellulose), affinity chromatography on Affi-gel blue gel, ion exchange chromatography on SP-sepharose (Sulfopropyl-sepharose), and gel filtration by FPLC (Fast protein liquid chromatography) on Superdex 75. It dose-dependently inhibited trypsin with an IC50 value of 0.4 mu M, and this activity was reduced in the presence of dithiothreitol in a dose- and time-dependent manner, signifying the importance of the disulfide linkage to the activity. It inhibited [methyl-H-3] thymidine incorporation by leukemia L1210 cells and lymphoma MBL2 cells with an IC50 value of 2.3 mu M and 2.5 mu M, respectively. The inhibitor had no effect on fungal growth and the activities of various viral enzymes when tested up to 100 mu M.

分类号： O62

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