Immortalized bovine mammary epithelial cells express stem cell markers and differentiate in vitro
文献类型: 外文期刊
第一作者: Hu, Han
作者: Hu, Han;Zheng, Nan;Hu, Han;Gao, Haina;Dai, Wenting;Zhang, Yangdong;Li, Songli;Wang, Jiaqi
作者机构:
关键词: bovine mammary epithelial cell;differentiation;immortalization;stem cell
期刊名称:CELL BIOLOGY INTERNATIONAL ( 影响因子:3.612; 五年影响因子:3.055 )
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年卷期:
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收录情况: SCI
摘要: The bovine mammary epithelial cell is a secretory cell, and its cell number and secretory activity determine milk production. In this study, we immortalized a bovine mammary epithelial cell line by SV40 large T antigen gene using a retrovirus based on Chinese Holstein primary mammary epithelial cells (CMEC) cultured in vitro. An immortalized bovine mammary epithelial cell line surpassed the 50-passage mark and was designated the CMEC-H. The immortalized mammary epithelial cells grew in close contact with each other and exhibited the typical cobblestone morphology characteristic with obvious boundaries. The telomerase expression of CMEC-H has consistently demonstrated the presence of telomerase activity as an immortalized cell line, but the cell line never induced tumor formation in nude mice. CMEC-H expressed epithelial (cytokeratins CK7, CK8, CK18, and CK19), mesenchymal (vimentin), and stem/progenitor (CD44 and p63) cell markers. The induced expression of milk proteins, (S1)-casein, -casein, -casein, and butyrophilin, indicated that CMEC-H maintained the synthesis function of the mammary epithelial cells. The established immortalized bovine mammary epithelial cell line CMEC-H is capable of self-renewal and differentiation and can serve as a valuable reagent for studying the physiological mechanism of the mammary gland.
分类号: Q2
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