Raoultella sp strain L03 fixes N-2 in association with micropropagated sugarcane plants
文献类型: 外文期刊
第一作者: Yang, Li-Tao
作者: Yang, Li-Tao;Li, Yang-Rui;Zhang, Ge-Min;Gao, Yi-Jing;Duan, Wei-Xing;Luo, Ting;Ou-Yang, Xue-Qing;Yang, Li-Tao;Li, Yang-Rui;Song, Xiu-Peng;Ou-Yang, Xue-Qing;An, Qianli
作者机构:
关键词: Endophytic bacteria;Nitrogen fixation;Plant growth;promoting bacteria;Raoultella;Sugarcane
期刊名称:JOURNAL OF BASIC MICROBIOLOGY ( 影响因子:2.281; 五年影响因子:2.516 )
ISSN:
年卷期:
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收录情况: SCI
摘要: N-2-fixing bacteria belonging to the genus Raoultella of the family Enterobacteriaceae are widely associated with plants. Raoultella sp. strain L03 was isolated from surface-sterilized sugarcane roots. In this study, we inoculated the strain L03 to microbe-free micropropagated plantlets of the main sugarcane cultivar ROC22 grown in Guangxi, China and determined z-fixation and association between strain L03 and sugarcane plants. Inoculation of strain L03 increased plant biomass, total N, N concentration and chlorophyll, and relieved N-deficiency symptoms of plants under an N-limiting condition. An 15N isotope dilution assay revealed 15N isotope dilution in the inoculated sugarcane plants and incorporation of the fixed 14N from air into chlorophyll. Moreover, a gfp-tagged and antibiotic-resistant L03 strain was reisolated from surface-sterilized sugarcane plants and was detected in plant tissues by fluorescent microscopy. This study for the first time demonstrates that a Raoultella bacterium is able to fix N-2 in association with the plant host.
分类号: R37
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