Investigating the mechanisms of glyphosate resistance in goosegrass (Eleusine indica (L.) Gaertn.) by RNA sequencing technology
文献类型: 外文期刊
第一作者: Huang, Hongjuan
作者: Huang, Hongjuan;Wei, Shouhui;Huang, Zhaofeng;Wang, Xu;Zhang, Chaoxian
作者机构:
关键词: transcriptomics;next-generation sequencing;herbicide resistance;glyphosate;(Eleusine indica(L.)Gaertn.)
期刊名称:PLANT JOURNAL ( 影响因子:6.417; 五年影响因子:7.627 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Glyphosate is an important non-selective herbicide that is in common use worldwide. However, evolved glyphosate-resistant (GR) weeds significantly affect crop yields. Unfortunately, the mechanisms underlying resistance in GR weeds, such as goosegrass (Eleusine indica (L.) Gaertn.), an annual weed found worldwide, have not been fully elucidated. In this study, transcriptome analysis was conducted to further assess the potential mechanisms of glyphosate resistance in goosegrass. The RNA sequencing libraries generated 24 597 462 clean reads. De novo assembly analysis produced 48 852 UniGenes with an average length of 847 bp. All UniGenes were annotated using seven databases. Sixteen candidate differentially expressed genes selected by digital gene expression analysis were validated by quantitative real-time PCR (qRT-PCR). Among these UniGenes, the EPSPS and PFK genes were constitutively up-regulated in resistant (R) individuals and showed a higher copy number than that in susceptible (S) individuals. The expressions of four UniGenes relevant to photosynthesis were inhibited by glyphosate in S individuals, and this toxic response was confirmed by gas exchange analysis. Two UniGenes annotated as glutathione transferase (GST) were constitutively up-regulated in R individuals, and were induced by glyphosate both in R and S. In addition, the GST activities in R individuals were higher than in S. Our research confirmed that two UniGenes (PFK,EPSPS) were strongly associated with target resistance, and two GST-annotated UniGenes may play a role in metabolic glyphosate resistance in goosegrass.
分类号: Q94
- 相关文献
作者其他论文 更多>>
-
A synthetic antibiotic class with a deeply-optimized design for overcoming bacterial resistance
作者:Feng, Jin;Peng, Dapeng;Wang, Xu;Feng, Jin;Weng, Defeng;Wang, Xu;Zheng, Youle;Ma, Wanqing;Weng, Defeng;Peng, Dapeng;Wang, Xu;Xu, Yindi;Wang, Zhifang
关键词:
-
Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland
作者:Wang, Ziqi;An, Yixin;Li, Ying;Yu, Qiang;Wang, Jie;Wang, Xu;Wu, Honghui;Yang, Tian;Zhang, Yunlong;Bian, Jianlin;Ren, Haiyan;Lkhagva, Ariuntsetseg
关键词:nitrogen deposition; C:N:P stoichiometry; grassland ecosystem; community level; belowground
-
Phased gap-free genome assembly of octoploid cultivated strawberry illustrates the genetic and epigenetic divergence among subgenomes
作者:Song, Yanhong;Liu, Lifeng;Li, Gang;Zhao, Xia;Zhou, Houcheng;Peng, Yanling;Wang, Xu;Cao, Shuo;Zhou, Yongfeng;Muyle, Aline;Zhou, Yongfeng
关键词:
-
Genomic Insights into Seed Germination Differences in Buffalobur (Solanum rostratum Dunal) under Contrasting GA and ABA Availability
作者:Chen, Zhaoxia;Li, Longlong;Wu, Kaidie;Zhao, Dandan;Yang, Long;Huang, Hongjuan;Huang, Zhaofeng;Wei, Shouhui
关键词:seed dormancy and germination; RNA-Seq; phytohormone; endo-beta-mannanase; endosperm cap
-
pOsHAK1:OsSUT1 Promotes Sugar Transport and Enhances Drought Tolerance in Rice
作者:Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu;Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu;Chen, Guang;Lian, Wenli;Geng, Anjing;Wang, Yihan;Liu, Minghao;Zhang, Yue;Wang, Xu
关键词:rice; drought tolerance; sugar transport; inducible promoter
-
AflaILVB/G/I and AflaILVD are involved in mycelial production, aflatoxin biosynthesis, and fungal virulence in Aspergillus flavus
作者:Zhao, Yarong;Huang, Chulan;Zeng, Rui;Chen, Peirong;Xu, Kaihang;Huang, Xiaomei;Wang, Xu;Zhao, Yarong;Huang, Chulan;Zeng, Rui;Chen, Peirong;Xu, Kaihang;Huang, Xiaomei;Wang, Xu;Zhao, Yarong;Huang, Chulan;Zeng, Rui;Chen, Peirong;Xu, Kaihang;Huang, Xiaomei;Wang, Xu
关键词:Aspergillus flavus; aflatoxin biosynthesis; branched-chain amino acids; AflaILVB/G/I; AflaILVD; fungal secondary metabolites
-
Spatiotemporal patterns and driving factors of gross primary productivity over the Mongolian Plateau steppe in the past 20 years
作者:Ding, Lei;Shi, Zhou;Chang, Jinfeng;Li, Zhenwang;Li, Zhenwang;Ding, Lei;Wang, Xu;Shen, Beibei;Shao, Changliang;Xiao, Liujun;Dong, Gang;Yu, Lu;Yu, Lu;Nandintsetseg, Banzragch;Nandintsetseg, Banzragch
关键词:Gross primary productivity; Mongolian Plateau; Grassland; Climatic factors; Human activity