Characterization and distinction of two flagellar systems in extraintestinal pathogenic Escherichia coli PCN033

文献类型： 外文期刊

第一作者： Liu, Feng

作者： Liu, Feng;Fu, Jiyang;Chen, Jing;Chen, Huanchun;Tan, Chen;Wang, Xiangru;Liu, Feng;Fu, Jiyang;Chen, Jing;Chen, Huanchun;Tan, Chen;Wang, Xiangru;Liu, Canying;Sun, Minhua;Chen, Huanchun;Tan, Chen;Wang, Xiangru

作者机构：

关键词： Flagella;Flag-1;Flag-2;ExPEC;flgD;IfgD

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.415； 五年影响因子：6.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Extraintestinal pathogenic Escherichia coli (ExPEC) can invade and colonize multiple extraintestinal tissues and can cause a wide range of infections; however the mechanisms of its pathogenicity are not well understood. Flagella contribute to the infection of E. coli strains by mediating adhesion and invasion. Our previous bioinformatic analysis revealed two flagella gene clusters in the genome of an ExPEC isolate, PCN033. One encodes the conventional flagellum system (Flag-1) and the other encodes the Flag-2 system, whose function is uncharacterized. Here we aimed to characterize these two flagellum systems and determine their contributions to the flagellum formation and certain pathogenicity-associated phenotypes. Our observations support the involvement of Flag-1 system, but not Flag-2 system, in the synthesis and maturation of the flagellum structure, and in mediating bacterial swimming and swarming. Moreover, flgD, which encodes a flagellar-hook scaffolding protein in the Flag-1 system, is required for flagellum assembly by influencing the production of FliC (flagellin). Deletion of flgD attenuated ExPEC strain PCN033 invasion and colonization in vivo, probably by affecting bacterial adhesion and invasion, and by reducing resistance to phagocytosis by circulating monocytes. In contrast, these phenotypes were not observed in the strain with deletion of lfgD, encoding the FlgD-like protein in the Flag-2 system. Taken together, these findings indicate that Flag-1 flagellum system is the determinative component of bacterial flagella that contributes to the infection. (C) 2016 Elsevier GmbH. All rights reserved.

分类号： Q

  • 相关文献
作者其他论文 更多>>

微信小程序