Graphene oxide and carboxylated graphene oxide: Viable two-dimensional nanolabels for lateral flow immunoassays

文献类型： 外文期刊

第一作者： Yu, Li

作者： Yu, Li;Li, Peiwu;Ding, Xiaoxia;Zhang, Qi;Yu, Li;Ding, Xiaoxia;Zhang, Qi;Li, Peiwu;Li, Peiwu;Ding, Xiaoxia;Yu, Li;Li, Peiwu

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关键词： Graphene oxide;Immunochromatographic assay;Aflatoxin B-1;Label;Screening;Food analysis

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

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年卷期：

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收录情况： SCI

摘要： Graphene oxide (GO) and carboxylated GO were used as labels for lateral flow immunoassays, instead of the conventionally used colloidal gold and colored latex labels. A sensor is demonstrated that enables fast screening for aflatoxin B-1 (AFB(1)) as a model analyte using the antibody-GO complex as the recognition element. The visual limit of detection and cut-off value for AFB1 are 0.3 and 1 ng/mL, respectively. It is shown that GO and carboxylated GO are viable black labels for use in lateral flow assays, one typical advantage being the saving cost (compared to the use of colloidal gold). Qualitative results are achieved within 15 min, and the analytical results were in good agreement with the reference LC MS/MS method. The method was successfully applied to the on site determination of AFB(1) in agricultural products. In our perception, it opens new possibilities for the screening of other toxins by lateral flow immunoassays using GO and carboxylated GO as labels.

分类号： O65

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