Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.)

文献类型： 外文期刊

第一作者： Li, Zhenyi

作者： Li, Zhenyi;Long, Ruicai;Zhang, Tiejun;Wang, Zhen;Zhang, Fan;Yang, Qingchuan;Kang, Junmei;Sun, Yan

作者机构：

关键词： Heat shock protein;Medicago sativa;Stress response;Transgenic Arabidopsis

期刊名称：JOURNAL OF PLANT RESEARCH （ 影响因子：2.629； 五年影响因子：2.926 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) are a ubiquitously expressed class of protective proteins that play a key role in plant response to stressful conditions. This study aimed to characterize and investigate the function of an HSP gene in alfalfa (Medicago sativa). MsHSP70, which contains a 2028-bp open reading frame, was identified through homology cloning. MsHSP70 shares high sequence identity (94.47%) with HSP70 from Medicago truncatula. Expression analysis of MsHSP70 in alfalfa organs revealed a relatively higher expression level in aerial organs such as flowers, stems and leaves than in roots. MsHSP70 was induced by heat shock, abscisic acid (ABA) and hydrogen peroxide. Transgenic Arabidopsis seedlings overexpressing MsHSP70 were hyposensitive to polyethylene glycol (PEG) and ABA treatments, suggesting that exogenous expression of MsHSP70 enhanced Arabidopsis tolerance to these stresses. Examination of physiological indexes related to drought and ABA stress demonstrated that in comparison with non-transgenic plants, T3 transgenic Arabidopsis plants had an increased proline content, higher superoxide dismutase (SOD) activity, and decreased malondialdehyde (MDA) content. Furthermore, higher relative water content (RWC) was detected in transgenic plants compared with non-transgenic plants under drought stress. These findings clearly indicate that molecular manipulation of MsHSP70 in plants can have substantial effects on stress tolerance.

分类号： Q94

  • 相关文献

[1]Molecular cloning and characterization of the MsHSP17.7 gene from Medicago sativa L.. Li, Zhen-yi,Long, Rui-cai,Zhang, Tie-jun,Yang, Qing-chuan,Kang, Jun-mei.

[2]Functional identification of apple MdJAZ2 in Arabidopsis with reduced JA-sensitivity and increased stress tolerance. An, Xiu-Hong,Li, En-Mao,Xu, Kai,Cheng, Cun-Gang,Hao, Yu-Jin.

[3]Induction of heme oxygenase-1 with beta-CD-hemin complex mitigates cadmium-induced oxidative damage in the roots of Medicago sativa. Fu, Guangqing,Cui, Weiti,Wang, Yanqin,Shen, Wenbiao,Zhang, Liefeng,Ren, Yong,Zheng, Tianqing. 2011

[4]Physiological and antioxidant responses of three leguminous species to saline environment during seed germination stage. Wang, Xiaoshan,Gu, Hongru,Wang, Xiaoshan,Zhao, Guoqi. 2009

[5]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[6]Effect of rhizobia symbiosis on lignin levels and forage quality in alfalfa (Medicago sativa L.). Zhang, Zhiqiang,Chang, Leqin,Cao, Yuman,Zhang, Tong,Wang, Yafang,Liu, Yushi,Zhang, Pan,Hu, Tianming,Yang, Peizhi,Zhang, Zhiqiang,Shao, Linhui,Zhang, Pan,Sun, Xiaoqin,Wu, Yajun.

[7]Methane alleviates alfalfa cadmium toxicity via decreasing cadmium accumulation and reestablishing glutathione homeostasis. Gu, Quan,Chen, Ziping,Cui, Weiti,Zhang, Yihua,Yu, Xiuli,Wang, Qingya,Shen, Wenbiao,Hu, Huali. 2018

[8]A rapid screening method for evaluating resistance of alfalfa (Medicago sativa L.) to Fusarium root rot. Cong, L. L.,Wang, Z.,Kang, J. M.,Zhang, T. J.,Yang, Q. C.,Cong, L. L.,Sun, Y.,Biligetu, B.. 2018

[9]Effects of dietary alfalfa flavonoids on the performance, meat quality and lipid oxidation of growing rabbits. Dabbou, Sihem,Schiavone, Achille,Gasco, Laura,Rotolo, Luca,Gasco, Laura,Schiavone, Achille,Gai, Francesco,Pozzo, Luisa,Tong, Jian Ming,Dong, Xiao Fang,Rubiolo, Patrizia. 2018

[10]Genetic diversity and phenotypic variation for drought resistance in alfalfa (Medicago sativa L.) germplasm collected for drought tolerance. Zhang, Tiejun,Kesoju, Sandya,Hu, Jinguo,Yu, Long-Xi,Zhang, Tiejun,Kesoju, Sandya,Greene, Stephanie L.,Fransen, Steven. 2018

[11]Expression of two uricase (Nodulin-35) genes in a non-ureide type legume, Medicago sativa. Cheng, XG,Nomura, M,Takane, K,Kouchi, H,Tajima, S. 2000

[12]Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana. Chao, Yuehui,Zhang, Tiejun,Yang, Qingchuan,Kang, Junmei,Qin, Zhihui,Sun, Yan,Gruber, Margaret Yvonne,Qin, Zhihui. 2014

[13]Genetic relationships among alfalfa gemplasms resistant to common leaf spot and selected Chinese cultivars assessed by sequence-related amplified polymorphism (SARP) markers. Yuan, Qinghua,Wang, Yu,Li, Xiang-lin,Gao, Jianming,Gui, Zhi,Wang, Shuang,Zhao, Ximan,Xia, Buxian. 2011

[14]Completion of the agronomic evaluations of Medicago ruthenica [(L.) Ledebour] germplasm collected in Inner Mongolia. Campbell, TA,Bao, G,Xia, ZL. 1999

[15]Protective function of narrow grass hedges on soil and water loss on sloping croplands in Northern China. Xiao, Bo,Wang, Qing-hai,Wu, Ju-ying,Huang, Chuan-wei,Yu, Ding-fang,Xiao, Bo.

[16]Allelopathic potential and volatile compounds of Manihot esculenta Crantz against weeds. Li, J.,He, S. Y.,Qin, X. D..

[17]Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii. Gong, Haiyan,Kobayashi, Kyousuke,Sugi, Tatsuki,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Horimoto, Taisuke,Akashi, Hiroomi,Kato, Kentaro,Gong, Haiyan,Sugi, Tatsuki,Takemae, Hitoshi,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Xuan, Xuenan,Kato, Kentaro. 2014

[18]Interaction of heat shock protein 90 B1 (Hsp90B1) with liposome reveals its potential role in protection the integrity of lipid membranes. Li, Pengpeng,Zhang, Muhan,Zou, Ye,Sun, Zhilan,Sun, Chong,Geng, Zhiming,Xu, Weimin,Wang, Daoying. 2018

[19]Biochemical and molecular responses of herbaceous peony to high temperature stress. Wu, Yan-Qing,Zhao, Da-Qiu,Han, Chen-Xia,Tao, Jun,Zhao, Da-Qiu,Tao, Jun. 2016

[20]3 '-epi-12 beta-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Mei, Wen-Li,Dai, Hao-Fu,Liu, Quan,Liu, Quan,Huang, Canhua,Li, Yue-Nan. 2018

作者其他论文 更多>>

微信小程序