A fully in vitro protocol towards large scale production of recombinant inbred lines in wheat (Triticum aestivum L.)

文献类型： 外文期刊

第一作者： Yao, Yan

作者： Yao, Yan;Zhang, Ping;Lu, Zhanyuan;Liu, Hui;Yan, Guijun;Liu, Hui;Yan, Guijun

作者机构：

关键词： Wheat;Triticum aestivum L.;Fast generation cycling;Single-seed-decent;Flowering and seed-set in vitro

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A bottleneck for genetic research and breeding of crop plants is the time taken to producing large pure line segregating populations so called recombinant inbred lines (RILs). One way to overcome this problem is through use of the single-seed-decent (SSD) process under in vitro conditions. A number of factors that may affect in vitro SSD approach of wheat including temperature, light duration and intensity, salt strength and carbohydrate concentration were investigated in this study. Under the in vitro conditions, 45 days per generation was achieved for an early flowering wheat genotype Emu Rock, allowing eight generations per annum; 58 days per generation was achieved for mid flowering genotypes, allowing six generations per annum. The results showed that a variation of growth environment before and after three-leaf stage allowed in vitro seed-set with a relatively short generation time. Specifically, the plantlets were first grown under 22 A degrees C with a light intensity of 145 mu mol m(-2) s (-1) (16 h d(-1)) for 20 days (around three-leaf stage), and then moved to an environment of 28 A degrees C and 500 mu mol m(-2) s(-1) (20 h d(-1)) light. The culture medium was 1/2 strength Murashige and Skoog (MS) with modification of adding ten times of extra KH2PO4 and 4% sucrose. The fully in vitro protocol resulted in 100% flowering rate and average seed set rate of 91.5% in Emu Rock and Zippy. It can be further fine-tuned to suit different genotypes and it has a potential for factory scale mass-production of RILs for genetic studies and practical breeding programs.

分类号： Q942

  • 相关文献

[1]Allelopathic effects of aerial parts of Descurainia sophia L. on wheat. Liu, X. G.,Lu, C. H.,Dong, F. S.,Xu, J.,Wu, Y. B.,Zheng, Y. Q.,Tian, F. J.,Wu, Y. B.. 2016

[2]Improvement of resistance to Fusarium head blight by recurrent selection in an intermating breeding spring wheat population using the dominant male-sterile gene ms(2). Yang, ZP,Yang, XY,Huang, DC. 2000

[3]Crop production, nitrogen recovery and water use efficiency in rice-wheat rotation as affected by non-flooded mulching cultivation (NFMC). Ai, YW,Zhang, FS,Lu, SH,Zeng, XZ,Fan, MS. 2005

[4]Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties. Miao, Jun,Sun, Jinghan,Liu, Dongcheng,Li, Bin,Zhang, Aimin,Li, Zhensheng,Tong, Yiping,Miao, Jun. 2009

[5]A genotypic difference in primary root length is associated with the inhibitory role of transforming growth factor-beta receptor-interacting protein-1 on root meristem size in wheat. He, Xue,Fang, Jingjing,Li, Jingjuan,Qu, Baoyuan,Ren, Yongzhe,Ma, Wenying,Zhao, Xueqiang,Li, Bin,Wang, Daowen,Li, Zhensheng,Tong, Yiping,Fang, Jingjing,Li, Jingjuan,Ren, Yongzhe. 2014

[6]QTL Mapping for Adult Plant Resistance to Powdery Mildew in Italian Wheat cv. Strampelli. Asad Muhammad Azeem,BAI Bin,LAN Cai-xia,YAN Jun,XIA Xian-chun,ZHANG Yong,HE Zhong-hu. 2013

[7]Molecular detection of high- and low-molecular-weight glutenin subunit genes in common wheat cultivars from 20 countries using allele-specific markers. H. Jin,J. Yan,R. J. Peña,X. C. Xia,A. Morgounov,L. M. Han,Y. Zhang,Z. H. He. 2011

[8]The gluten protein and interactions between components determine mixograph properties in an F-6 recombinant inbred line population in bread wheat. Yong Zhang,Jianwei Tang,Jun Yan,Yelun Zhang,Yan Zhang,Xianchun Xia,Zhonghu He. 2009

[9]A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. Liu, Li,Wang, Aili,Ma, Junhong,Lan, Ping,Ma, Wujun,Liu, Li,Xia, Xianchun,He, Zhonghu,Wang, Aili,Yan, Yueming,He, Zhonghu,Bekes, Frank,Appels, Rudi. 2009

[10]Uncovering Male Fertility Transition Responsive miRNA in a Wheat Photo-Thermosensitive Genic Male Sterile Line by Deep Sequencing and Degradome Analysis. Bai, Jian-Fang,Wang, Yu-Kun,Wang, Peng,Duan, Wen-Jing,Yuan, Shao-Hua,Sun, Hui,Yuan, Guo-Liang,Ma, Jing-Xiu,Wang, Na,Zhang, Feng-Ting,Zhang, Li-Ping,Zhao, Chang-Ping,Bai, Jian-Fang,Wang, Yu-Kun,Wang, Peng,Duan, Wen-Jing,Yuan, Shao-Hua,Sun, Hui,Yuan, Guo-Liang,Ma, Jing-Xiu,Wang, Na,Zhang, Feng-Ting,Zhang, Li-Ping,Zhao, Chang-Ping,Wang, Peng,Duan, Wen-Jing. 2017

[11]Virus-Based MicroRNA Silencing and Overexpressing in Common Wheat (Triticum aestivum L.). Jian, Chao,Chi, Qing,Wang, Shijuan,Ma, Meng,Liu, Xiangli,Zhao, Huixian,Han, Ran,Zhao, Huixian. 2017

[12]A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat. Wang Yi-xue,Li Run-zhi,Xu Qiao-fang,Chang Xiao-ping,Hao Chen-yang,Jing Rui-lian. 2018

[13]GENETIC ANALYSIS OF THE GRAIN PROTEIN CONTENT IN SOFT RED WINTER WHEAT (Triticum aestivum L.). Yao, Jinbao,Ma, Hongxiang,Yang, Xueming,Zhou, Miaoping,Yang, Dan. 2014

[14]Genetic analysis of plant height and its components in diallel crosses of bread wheat (Triticum aestivum L.). Yao, J. B.,Ma, H. X.,Ren, L. J.,Zhang, P. P.,Yang, X. M.,Yao, G. C.,Zhang, P.,Zhou, M. P.. 2011

[15]What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting?. Xia, L. Q.,Yang, Y.,Ma, Y. Z.,Chen, X. M.,He, Z. H.,Roeder, M. S.,Jones, H. D.,Shewry, P. R.. 2009

[16]Agronomic improvements through the genetic and physiological regulation of nitrogen uptake in wheat (Triticum aestivum L.). Kong, Lingan,Wang, Fahong,Si, Jisheng,Lopez-bellido, Luis,Si, Jisheng,Maria Garcia-mina, Jose,Si, Jisheng. 2013

[17]Identification of SSR Marker Linked to a Major Dwarfing Gene in Common Wheat. Meng Ya-ning,Kang Su-hua,Lan Su-que,Li Xing-pu,Zhang Ye-lun,Bai Feng. 2013

[18]Evolutionary and functional study of the CDPK gene family in wheat (Triticum aestivum L.). Li, Ai-Li,Zhu, Yuan-Fang,Tan, Xiao-Mei,Wang, Xiang,Wei, Bo,Guo, Han-Zi,Zhang, Zeng-Lin,Chen, Xiao-Bo,Zhao, Guang-Yao,Kong, Xiu-Ying,Jia, Ji-Zeng,Mao, Long,Tan, Xiao-Mei.

[19]Differential responses of root and root hair traits of spring wheat genotypes to phosphorus deficiency in solution culture. Wang, Y. S.,Wang, Y. S.,Jensen, L. S.,Magid, J..

[20]Effects of the Vrn-D1b allele associated with facultative growth habit on agronomic traits in common wheat. Meng, Ling-zhi,Liu, Hong-wei,Yang, Li,Li, Hong-jie,Zhang, Hong-jun,Zhou, Yang,Mai, Chun-yan,Yu, Li-qiang.

作者其他论文 更多>>

微信小程序