Successful detection of foreign inserts in transgenic rice TT51-1 (BT63) by RNA-sequencing combined with PCR

文献类型: 外文期刊

第一作者: Li, Yunjing

作者: Li, Yunjing;Li, Jun;Wu, Yuhua;Cao, Yinglong;Zhu, Li;Li, Xiaofei;Huang, Shunmou;Wu, Gang;Li, Yunjing;Li, Jun;Wu, Yuhua;Cao, Yinglong;Zhu, Li;Li, Xiaofei;Wu, Gang

作者机构:

关键词: genetically modified organism;TT51-1;Solexa sequencing;PCR

期刊名称:JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE ( 影响因子:3.638; 五年影响因子:3.802 )

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收录情况: SCI

摘要: BACKGROUND: As event-specific sequence information for most unauthorised genetically modified organisms (GMOs) is currently still unavailable, detecting unauthorised GMOs remains challenging. Here, we used insect-resistant rice TT51-1 as an example to develop a novel approach via detecting GMOs by RNA-seq (sequencing) and PCR. RNA-seq of TT51-1 generated 4.8 million (M) 21-nt cDNA tags. Alignment to the Oryza sativa subsp. japonica reference genome revealed 24 098 unmapped tags. Foreign tags from the nopaline synthetic enzyme gene (NOS) terminator and insect-resistant genes were then identified by searching against the NCBI VecScreen and NT databases.

分类号: S

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