Poly(A) polymerase I participates in the indole regulatory pathway of Pantoea agglomerans YS19

文献类型： 外文期刊

第一作者： Li, Zihua

作者： Li, Zihua;Jiang, Jing;Yu, Xuemei;Feng, Yongjun;Wu, Cunxiang;Shen, Delong

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关键词： Pantoea agglomerans YS19;indole;poly(A) polymerase;symplasmata;Tn5 transposon

期刊名称：MICROBIOLOGY-SGM （ 影响因子：2.777； 五年影响因子：2.871 ）

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收录情况： SCI

摘要： Pantoea agglomerans YS19 is a preponderant endophytic bacterium isolated from rice. It is characterized by the formation of symplasmata, a type of multicellular aggregate structure, contributing to a strong stress resistance and specific adaptation of YS19 in endophyte-host associations. Indole is an important signal molecule in intra- or interspecies relationships, regulating a variety of bacterial behaviours such as cell aggregation and stress resistance; however, the regulatory mechanism remains an ongoing area of investigation. This study selected YS19 as a model strain to construct a mutant library, utilizing the mTn5 transposon mutagenesis method, thus obtaining a positive mutant with an indole-inhibited mutation gene. Via thermal asymmetric interlaced PCR, the mutational site was identified as the gene of pcnB, which encodes the poly(A) polymerase I to catalyse the polyadenylation of RNAs. The full length of the pcnB sequence was 1332 bp, and phylogenetic analysis revealed that pcnB is extremely conserved among strains of P. agglomerans. The expression of the gene was significantly inhibited (by 36.6% as detected via quantitative PCR) by indole (0.5 mM). Many physiological behaviours of YS19 were affected by this mutation: the cell decay rate in the post-stationary growth phase was promoted, symplasmata formation and motility were inhibited in the late stationary growth phase and the colonization ability and growth-promoting effect of YS19 on the host plant were also inhibited. This study discusses the indole regulatory pathways from the point of RNA post-transcriptional modification, thus enriching our knowledge of polyadenylation and expanding current research ideas of indole regulation.

分类号： Q93

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[1]Indole affects the formation of multicellular aggregate structures in Pantoea agglomerans YS19. Yu, Xuemei,Jiang, Jing,Liang, Chen,Zhang, Xiao,Wang, Jieru,Feng, Yongjun,Shen, Delong.

[2]Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites. Jia, Xianbo,Lin, Xinjian,Chen, Jichen,Jia, Xianbo. 2017

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