Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China
文献类型: 外文期刊
第一作者: Fan, Baochao
作者: Fan, Baochao;Yu, Zhengyu;Pang, Fengjiao;Xu, Xiangwei;Zhang, Baimeng;Guo, Rongli;He, Kongwang;Li, Bin;Fan, Baochao;Yu, Zhengyu;Pang, Fengjiao;Xu, Xiangwei;Zhang, Baimeng;Guo, Rongli;He, Kongwang;Li, Bin
作者机构:
关键词: PEDV;Isolation;Reverse genetics;Highly pathogenic
期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Since 2010, outbreaks of variant porcine epidemic diarrhea virus (PEDV) have swept across the world causing substantial economic losses. The development of new, more effective vaccines has been hampered by difficulties in isolating strains and viral genome manipulation. In the present study, we successfully isolated a highly pathogenic field strain AH2012/12, from a pig farm reporting severe diarrhea in China. Phylogenetic analysis revealed that the new isolate belongs to group G2, which represents epidemic and pandemic field strains. Furthermore, we constructed an infectious cDNA clone of the newly isolated strain, rAH2012/12, and the rescued virus displayed phenotypic properties identical to the parental virus in vitro. In vivo experiments demonstrated that the rescued virus displayed similar pathogenicity to the parental isolate, causing high mortality rates in suckling pigs. This study provided a strong basis for the development of live attenuated vaccines and for research into the pathogenic mechanisms of this virus.
分类号: R37
- 相关文献
作者其他论文 更多>>
-
Combined detection of foodborne pathogens in irrigation water in Jiangsu, China
作者:Xie, Yajing;Zhang, Cunzheng;Liu, Xianjin;Xie, Yajing;Lu, Jiayao;Liu, Xianjin;Li, Bin;Fan, Bochao;Li, Dandi;Du, Xuefei;Xie, Yajing;Schmidt, Stefan
关键词:Irrigation water; Foodborne pathogens; Combined detection; Noroviruses; Strawberry production
-
Exploration of the fitting of Peleg equation and its application in the semidry milling of the glutinous brown rice
作者:Wang, Guozhen;Zhou, Jiejun;Li, Bin;Lyu, Qingyun;Ding, Wenping;Luo, Xiaogang;Tong, Li-Tao
关键词:Peleg equation; GBR; Semidry milling
-
Advancing Loquat Total Soluble Solids Content Determination by Near-Infrared Spectroscopy and Explainable AI
作者:Luo, Yizhi;Lu, Huazhong;Qiu, Guangjun;Qi, Haijun;Li, Bin;Zhou, Xingxing;Jin, Qingting;Li, Peng
关键词:total soluble solids content; loquat; near-infrared spectroscopy; explainable artificial intelligence
-
Interactions, properties and lipid digestibility of attractive Pickering emulgels formed by sequential addition of oppositely charged nanopolysaccharides
作者:Guo, Shasha;Ji, Xingxiang;Guo, Shasha;Li, Jun;Huang, Luyao;Guo, Shasha;Wan, Zhangmin;Niu, Xun;Xu, Junhua;Liu, Ying;Bai, Long;Lu, Yi;Rojas, Orlando J.;Jiao, Wenjuan;Zheng, Jianan;Li, Bin;Bai, Long;Lu, Yi;Rojas, Orlando J.;Rojas, Orlando J.
关键词:
-
Dried Apricot Polyphenols Suppress the Growth of A549 Human Lung Adenocarcinoma Cells by Inducing Apoptosis via a Mitochondrial-Dependent Pathway
作者:Zhao, Caiyun;Wang, Jingteng;Guo, Jintian;Gao, Wenjing;Li, Bin;Shang, Xin;Zheng, Li;Fu, Yinghua;Wu, Bin
关键词:dried apricot polyphenols; A549; inhibition; apoptosis
-
Geographical origin traceability of Xiaobai apricot (Prunus armeniaca L.cv.) using stable isotope and multi-element analysis
作者:Hu, Yunsha;Liu, Huaying;Bai, Baowei;He, Weizhong;Shen, Qi;Chen, Chang;Tian, Jing;Mei, Xianxian;Li, Bin;Yu, Shuqi;Liu, Zhi;Liu, Zhi
关键词:Xiaobai apricot; Stable isotope; Mineral element; Rare earth element; Origin traceability
-
Molecular characteristics of the immune escape of coronavirus PEDV under the pressure of vaccine immunity
作者:Li, Yunchuan;Yang, Shanshan;Qian, Jiali;Liu, Shiyu;Li, Yupeng;Song, Xu;Cao, Qiuxia;Guo, Rongli;Zhao, Yongxiang;Sun, Min;Hu, Mi;Li, Jizong;Zhang, Xuehan;Fan, Baochao;Li, Bin;Li, Yunchuan;Yang, Shanshan;Qian, Jiali;Liu, Shiyu;Li, Yupeng;Song, Xu;Cao, Qiuxia;Guo, Rongli;Zhao, Yongxiang;Sun, Min;Hu, Mi;Li, Jizong;Zhang, Xuehan;Fan, Baochao;Li, Bin;Fan, Baochao;Fan, Baochao;Li, Bin;Li, Bin
关键词:coronavirus; PEDV; immune escape; immune pressure; S protein