Effective degradation of aflatoxin B-1 using a novel thermophilic microbial consortium TADC7
文献类型: 外文期刊
第一作者: Wang, Yi
作者: Wang, Yi;Zhao, Chunxia;Zhao, Mingming;Zheng, Dan;Cheng, Wei;Guo, Peng;Wang, Yi;Zhao, Chunxia;Lyu, Yucai;Zhang, Dongdong;Cui, Zongjun
作者机构:
关键词: Aflatoxin B1;Biodegradation;Microbial consortium;Thermophilic
期刊名称:BIORESOURCE TECHNOLOGY ( 影响因子:9.642; 五年影响因子:9.237 )
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收录情况: SCI
摘要: We constructed a novel thermophilic microbial consortium, TADC7, with stable and efficient aflatoxin B-1 (AFB(1)) degradation activity. The microbial consortium degraded more than 95% of the toxin within 72 h when cultured with AFB(1), and the optimum temperature was 55-60 degrees C. TADC7 tolerated high doses of AFB(1), with no inhibitory effects up to 5000 mu g L (1) AFB(1); moreover, the degradation kinetics fit well with the Monod model. The proteins or enzymes in the TADC7 cell-free supernatant played a major role in AFB(1) degradation. AFB1 degradation by the cell-free supernatant was stable up to 90 degrees C, with an optimal pH of 8-10. We performed 16S rRNA sequencing to determine TADC7 community structure dynamics; the results indicated that Geobacillus and Tepidimicrobium played major roles in AFB1 degradation. (C) 2016 Elsevier Ltd. All rights reserved.
分类号: Q
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