Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus

文献类型: 外文期刊

第一作者: Li, Qinghe

作者: Li, Qinghe;Yuan, Xiaoya;Wang, Qiao;Wang, Fei;Liu, Ranran;Zheng, Maiqing;Wen, Jie;Zhao, Guiping;Yuan, Xiaoya;Chang, Guobin;Chen, Guohong

作者机构:

关键词: H5N1 avian influenza A virus;PA-X;Chicken;Interactome

期刊名称:JOURNAL OF PROTEOMICS ( 影响因子:4.044; 五年影响因子:4.02 )

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收录情况: SCI

摘要: As a newly identified isoform generated from the PA segment and an essential factor for viral virulence, little is known about PA-X-host interactions. Here we present the interactomic landscape of PA-X protein of H5N1 influenza A virus (IAV), described using data generated from affinity purification and mass spectrometry (AP-MS). PA-X was exogenously expressed in chicken fibroblast cells and PA-X associated protein complexes were identified by AP-MS. Using a high confidence threshold for interaction 56 unique proteins were found to have physical interactions with PA-X. PA-X associated host factors showed strong enrichment for specific protein domains, including annexin and WD40 domains. Many proteins that have been described as pro or antiviral host proteins interact with PA-X, indicating the possible effect of these proteins on influenza A viral infections facilitated by interactions with PA-X. This study has uncovered the comprehensive interactomic landscape of PA-X and laid the foundation for further understanding of PA-X function in terms of viral-host protein interactions.

分类号: Q51

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