Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella

文献类型： 外文期刊

第一作者： Hu, Rong

作者： Hu, Rong;Guo, Xinhong;Hu, Rong;Wang, Yonghong;Qu, Fufa;Tang, Jianzhou;Zhou, Yi;Lu, Shuangqing;Zhang, Jianshe;Liu, Zhen;Zhou, Zhigang

作者机构：

期刊名称：JOURNAL OF THE WORLD AQUACULTURE SOCIETY （ 影响因子：2.512； 五年影响因子：2.47 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： AlaSerCys Transporter 2 (ASCT2), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full-length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella, which encodes a 541-amino-acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio. ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real-time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5g/kg glutamine and 2.5g/kg glutamine dipeptide remarkably improved the expression of ASCT2. These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.

分类号： S96

  • 相关文献
作者其他论文 更多>>

微信小程序