Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

文献类型： 外文期刊

第一作者： Xu, Pengjun

作者： Xu, Pengjun;Yang, Xianming;Wu, Kongming;Xu, Pengjun;Graham, Robert I.;Wilson, Kenneth

作者机构：

关键词： Helicoverpa armigera;Iflavirus;RNA virus;Horizontal transmission;Vertical transmission;Tissue distribution

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 10(8) copies/mu l while vertical transmission efficiency was found to be relatively low (<28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera. (C) 2017 Elsevier Inc. All rights reserved.

分类号： Q959.1

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