Characterization of Polysaccharides from the Fruiting Bodies of Two Species of Genus Ganoderma (Agaricomycetes) and Determination of Water-Soluble beta-D-Glucan Using High-Performance Liquid Chromatography
文献类型: 外文期刊
第一作者: Liu, Yanfang
作者: Liu, Yanfang;Tang, Qingjiu;Yang, Yan;Zhou, Shuai;Wu, Di;Tang, Chuanhong;Zhang, Zhong;Yan, Mengqiu;Feng, Jie;Zhang, Jingsong;Liu, Yanfang
作者机构:
关键词: determination of beta-D-glucan contcnt;Ganderma spp.;HPSEC-MALLS-RI;medicinal mushrooms;polysaccharide
期刊名称:INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS ( 影响因子:1.921; 五年影响因子:1.879 )
ISSN:
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收录情况: SCI
摘要: Molecular weight (M-w) distributions of polysaccharides from the fruiting bodies of different Ganoderma lucidum strains and G. sinense were investigated and compared using high-pressure size exclusion chromatography/multiangle laser light scattering/refractive index analysis. Results showed that there were big differences in the M-w distributions and characteristics of polysaccharides from 2 species of Ganoderma. All tested G. lucidum materials exhibited similar polysaccharide distributions and similar characteristics for each fraction. The fraction with highest M-w (peak 1) was identified as beta-(1 -> 3)-linked D-glucan with (1 -> 6)-beta-D-glucopyranosyl side branches. G. sinense fruiting bodies did not include the beta-D-glucan when compared with G. lucidum. A high-pressure size exclusion chromatography method was developed and applied to determine the amount of high-M-w beta-D-glucan in G. lucidum fruiting bodies. Results indicated that there was no obvious relationship between beta-D-glucan content and the genetic similarity of G. lucidum. The strain labeled "Longzhi no. 2" was determined to possess the largest amount of beta-D-glucan: 8.2 mg/mL based on the dry weight of fruiting bodies. The beta-D-glucan content in the hot water extract of Longzhi no. 2 reached 17.05%. For the "Hunong no. 1" strain, the beta-D-glucan content in log-cultivated fruiting bodies was much higher than that in bag-cultivated ones. This method could be used to improve quality control of polysaccharides in G. lucidum.
分类号: Q94
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