Drebrin restricts rotavirus entry by inhibiting dynamin-mediated endocytosis

文献类型： 外文期刊

第一作者： Li, Bin

作者： Li, Bin;Ding, Siyuan;Feng, Ningguo;Mooney, Nancie;Ooi, Yaw Shin;Diep, Jonathan;Kelly, Marcus R.;Yasukawa, Linda L.;Jackson, Peter K.;Greenberg, Harry B.;Li, Bin;Ding, Siyuan;Feng, Ningguo;Yasukawa, Linda L.;Greenberg, Harry B.;Li, Bin;Ding, Siyuan;Feng, Ningguo;Yasukawa, Linda L.;Greenberg, Harry B.;Li, Bin;Mooney, Nancie;Kelly, Marcus R.;Jackson, Peter K.;Ren, Lili;Patton, John T.;Yamazaki, Hiroyuki;Shirao, Tomoaki

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关键词： drebrin;rotavirus;endocytosis

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

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收录情况： SCI

摘要： Despite the wide administration of several effective vaccines, rotavirus (RV) remains the single most important etiological agent of severe diarrhea in infants and young children worldwide, with an annual mortality of over 200,000 people. RV attachment and internalization into target cells is mediated by its outer capsid protein VP4. To better understand the molecular details of RV entry, we performed tandem affinity purification coupled with high-resolution mass spectrometry to map the host proteins that interact with VP4. We identified an actin-binding protein, drebrin (DBN1), that coprecipitates and colocalizes with VP4 during RV infection. Importantly, blocking DBN1 function by siRNA silencing, CRISPR knockout (KO), or chemical inhibition significantly increased host cell susceptibility to RV infection. Dbn1 KO mice exhibited higher incidence of diarrhea and more viral antigen shedding in their stool samples compared with the wild-type littermates. In addition, we found that uptake of other dynamin-dependent cargos, including transferrin, cholera toxin, and multiple viruses, was also enhanced in DBN1-deficient cells. Inhibition of cortactin or dynamin-2 abrogated the increased virus entry observed in DBN1-deficient cells, suggesting that DBN1 suppresses dynamin-mediated endocytosis via interaction with cortactin. Our study unveiled an unexpected role of DBN1 in restricting the entry of RV and other viruses into host cells and more broadly to function as a crucial negative regulator of diverse dynamin-dependent endocytic pathways.

分类号： N

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