Extensive characterization of a lentiviral-derived stable cell line expressing rabbit hemorrhagic disease virus VPg protein

文献类型: 外文期刊

第一作者: Zhu, Jie

作者: Zhu, Jie;Miao, Qiuhong;Tan, Yonggui;Guo, Huimin;Li, Chuanfeng;Chen, Zongyan;Liu, Guangqing

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关键词: VPg;Rabbit hemorrhagic disease virus;Cell line

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

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收录情况: SCI

摘要: Rabbit hemorrhagic disease virus (RHDV) is an important member of the caliciviridae family. Currently, no suitable tissue culture system is available for proliferating RHDV, which limits the study of its pathogenesis. To bypass this obstacle, we established a cell line, RK13-VPg, stably expressing the VPg gene with a lentivirus packaging system in this study. In addition, the recently constructed RHDV replicon in our laboratory provided an appropriate model for studying the pathogenesis of RHDV without in vitro RHDV propagation and culture. Using this RHDV replicon and RK13-VPg cell line, we further demonstrated that the presence of VPg protein is essential for efficient translation of an RHDV replicon. Therefore, the RK13-VPg cell line is a powerful tool for studying the replication and translation mechanisms of RHDV. (C) 2016 Elsevier B.V. All rights reserved.

分类号: R37

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