Facile preparation of magnetic carbon nanotubes-immobilized lipase for highly efficient synthesis of 1,3-dioleoyl-2-palmitoylglycerol-rich human milk fat substitutes

文献类型： 外文期刊

第一作者： Zheng, Mingming

作者： Zheng, Mingming;Wang, Shi;Xiang, Xia;Shi, Jie;Huang, Juan;Deng, Qianchun;Huang, Fenghong;Deng, Qianchun;Xiao, Junyong

作者机构：

关键词： 1;3-Dioleoyl-2-palmitoylglycerol;Structured lipid;Magnetic carbon nanotubes;Enzymatic acidolysis

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, Candida lipolytica lipase (CLL) was immobilized on magnetic multi-walled carbon nanotubes (mMWCNTs) via hydrophobic and cation-exchange interaction. The resultant immobilized CLL showed much better thermal stability, biocatalyst activity and easier recycling than the free form. A method for efficient enzymatic acidolysis of tripalmitin (PPP) with oleic acid (OA), to produce OPO-rich TAGs, was developed, using the immobilized CLL as the biocatalyst. Under optimized conditions (2% water, 20 mg/ml enzyme, 1:6 PPP/OA, 50 degrees C, 2 h), the content of OPO in the final product reached 46.5%. CLL@mMWCNTs had a better activity and manipulative stability than commercial lipases. More importantly, the feasibility of CLL@mMWCNTs was also validated in the practical production of OPO-rich TAGs, using lard and restructured palm oil as the raw material. These results suggest that CLL@mMWCNTs is a promising biocatalyst for the OPO-rich TAGs production and will be helpful for the infant formula industry. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： TS2`TS201.2

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