Anti-inflammatory and hepatoprotective effects of glycyrrhetinic acid on CCl4-induced damage in precision-cut liver slices from Jian carp (Cyprinus carpio var. jian) through inhibition of the nf-kB pathway

文献类型： 外文期刊

第一作者： Cao, Liping

作者： Cao, Liping;Ding, Weidong;Jia, Rui;Du, Jingliang;Wang, Tao;Zhang, Chunyun;Gu, Zhengyan;Yin, Guojun;Cao, Liping;Ding, Weidong;Jia, Rui;Du, Jingliang;Wang, Tao;Zhang, Chunyun;Gu, Zhengyan;Yin, Guojun

作者机构：

关键词： Glycyrrhetinic acid;Cyprinus carpio var. jian;Precision-cut liver slices;CCl4;Nuclear factor-kappa B;Inflammatory cytokines

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In order to evaluate the antioxidant and anti-inflammatory effects of glycyrrhetinic acid (GA) on carbon tetrachloride (CCl4)-induced damage in precision-cut liver slices (PCLS) from Jian carp (Cyprinus carpio. Jian), an acute liver damage model was established in this study. The viability of PCLS, levels of anti-oxidases in liver homogenates, expression of inflammation-related genes including nuclear factor-kappa B (nf-kappa B)/c-rel, inducible nitric oxide synthase (inos), interleukin-1 beta interleukin-6 (il-6) and interleukin-8 (il-8), and protein levels of (nf-kappa B)/c-rel in liver tissues were measured. The results showed that pretreatment of PCLS with GA at 5 and 10 mu g/mL for 6 h significantly inhibited the cytotoxicity of CCl4. GA attenuated CCl4-induced oxidative stress in PCLS through promoting the recovery of superoxide dismutase (SOD) and glutathione (GSH) levels, and inhibiting malondialdehyde (MDA) synthesis. In inflammatory response, GA at both 5 and 10 mu g/mL significantly inhibited the increase in mRNA levels of inflammatory cytokines including nf-kB/c-rel, inos, il-1 beta, il-6 and il-8, and the protein level of Nf-kB/C-rel induced by CCl4. Furthermore, treatment with pyrrolyl dithiocarbamate (PDTC, 4 mu g/mL), an inhibitor of nuclear transcription factor nf-kB, significantly inhibited nf-kB levels, and transcription of downstream cytokines inos, il-1 beta, il-6 and il-8, also the viability of PCLS was significantly increased. These results indicated that GA suppressed inflammation and reduced cytotoxicity by inhibiting the nf-kB signaling pathway, and plays a role in liver protection. (C) 2017 Published by Elsevier Ltd.

分类号： S9

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