Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis)

文献类型： 外文期刊

第一作者： Zhao, Manman

作者： Zhao, Manman;Liu, Hui;Deng, Zhi;Chen, Jiangshu;Yang, Hong;Li, Dejun;Zhao, Manman;Li, Huiping;Xia, Zhihui

作者机构：

关键词： Rubber tree;Polyamines;S-Adenosylmethionine decarboxylase;Translational regulation;Genomic structure

期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 影响因子：2.391； 五年影响因子：2.836 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： 5-Adenosylmethionine decarboxylase (SAMDC) is a key rate-limiting enzyme involved in polyamines biosynthesis, and it plays important roles in plant growth, development and stresses response. However, no SAMDC gene was reported in rubber tree. Here wereport characteristics of an SAMDC gene (HbSAMDCl) in rubber tree. HbSAMDCl contains a 1080 bp open reading frame (ORF) encoding 359 amino acids. Quantitative real-time PCR analyses revealed that HbSAMDCl exhibited distinct expression patterns in different tissues and was regulated by various stresses, including drought, cold, salt, wounding, and H_20_2 treatments. HbSAMDCl 5' untranslated region (UTR) contains a highly conserved overlapping tiny and small upstream ORFs (uORFs), encoding 2 and 52 aminoacid residues, respectively. No introns were located in the main ORF of HbSAMDCl, whereas two introns were found in the 5' UTR. In transgenic tobaccos, the highly conserved small uORF of HbSAMDCl is found to be responsible for translational repression ofdownstream (3-glucuronidase reporter. To our knowledge, this is the first report on molecular cloning, expression profiles, and 5' UTR characteristics of HbSAMDCl. These results lay solidfoundation for further elucidating HbSAMDCl function in rubber tree.

分类号： Q94

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