Isolation and characterization of monocyte/macrophage from peripheral blood of half smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Sha, Zhenxia

作者： Sha, Zhenxia;Chen, Yadong;Sha, Zhenxia;Wang, Linqing;Sun, Luming;Chen, Yadong;Li, Chen;Chen, Songlin;Zheng, Yuan;Li, Chen;Chen, Songlin;Xin, Mei

作者机构：

关键词： Cynoglossus semilaevis;Monocyte;Macrophage;Peripheral blood cell;Immune function

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In the present study, the peripheral blood cells of half smooth tongue sole (Cynoglossus semilaevis) were examined by blood smear under the light microscopy. The proportion of main types of blood cells are as following: erythrocyte occupied the majority (92.3%), followed by thrombocyte (4.15%), granulocyte (1.7%), lymphocyte (1.5%) and monocyte (0.3%), respectively. Meanwhile, the isolation method of monocytes was established, by density gradient centrifugation to isolate mononuclear leukocytes of peripheral blood. In primary culture, the monocytes were adhered to the bottom of the flask without feeder cells and separated easily with suspended leukocytes in the medium in 3 h. After suspended leukocytes were removed, the monocytes multiplied rapidly with the two doubly during the 24 h, then the cells proliferated and kept stable until 48 h. When co-cultured with suspended leukocytes after three days, the monocytes could derive to typical macrophages, of which the size enlarged significantly and showed various forms such as like fried eggs, and giant irregular shape with pseudopod because cells fusion or deformation occurred until macrophages died in about two weeks. Monocytes showed strong respiratory burst activity after treated with Phorbol ester PMA and challenged by bacteria respectively. In addition, macrophage of half smooth tongue sole had typical macrophage features such as phagocytic capability, positive esterase activity, and the considerable expression of M-CSFR, MHC-II, IL-6, IL-10, TNF and arginase genes. That arginase expression in macrophages (3d and 5d after differentiation) was upregulated fluctuant suggest that the cultivation was mixture of alternatively activated type macrophage (M2) in the majority while the classically activated type (M1) win the minority. Furthermore, MHC-II, M-CSFR and IL-6 were significantly induced following LPS challenge. Collectively, the present study will be useful for the study on half smooth tongue sole immune systems and immune function. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： S9

  • 相关文献

[1]ALV-J infection induces chicken monocyte death accompanied with the production of IL-1 beta and IL-18. Dai, Manman,Liao, Ming,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Shi, Meiqing. 2017

[2]Chicken embryo extracts enhance spleen lymphocyte and peritoneal macrophages function. Su, Yujie,Yang, Yanjun,Li, Xi,Sun, Jun. 2012

[3]Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study. Qin, L.,Xu, J.,Wang, Y. G.. 2014

[4]Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch. Cheng, Anwei,Jin, Zhengyu,Xu, Xueming,Wan, Fachun,Wang, Jiaqi. 2008

[5]Endotoxin activates de novo sphingolipid biosynthesis via nuclear factor kappa B-mediated upregulation of Sptlc2. Chang, Zhi-Qiang,Lee, Su-Yeon,Kim, Hye-Jin,Kim, Jung Ran,Kim, Su-Jung,Hong, In-Kyung,Oh, Byung-Chul,Choi, Cheol-Soo,Park, Tae-Sik,Chang, Zhi-Qiang,Goldberg, Ira J.. 2011

[6]Oligochitosan stimulated phagocytic activity of macrophages from blunt snout bream (Megalobrama amblycephala) associated with respiratory burst coupled with nitric oxide production. Liu, Lichun,Zhou, Yang,Zhao, Xiaoheng,Wang, Hong,Wang, Li,Yuan, Gailing,Asim, Muhammad,Liu, Xiaoling,Lin, Li,Wang, Weimin,Lin, Li,Wang, Weimin,Lin, Li,Zeng, Lingbing,Lin, Li.

[7]Characterisation and immunostimulatory activity of an alpha-(1 -> 6)-D-glucan from the cultured Armillariella tabescens mycelia. Yu, Mengyao,Yang, Zhirong,Zheng, Linyong,Xu, Xiaoyan,Yu, Mengyao,Zheng, Linyong. 2008

[8]Preliminary Investigation of the Amount, the Molecular Weight and the Activity of Polysaccharides from Chaenomeles Speciosa Fruits in Ethanol Fractional Precipitation. Tian Bing-mei,Xie Xiao-mei,Shen Pan-pan,Yang Mo,Zhang Sheng-long,Xie Xiao-mei,Tang Qing-jiu. 2015

[9]Foot-and-mouth disease virus infection stimulates innate immune signaling in the mouse macrophage RAW 264.7 cells. Zhi, Xiaoying,Gao, Yuan,Wu, Run,Zhi, Xiaoying,Lv, Jianliang,Wei, Yanquan,Du, Ping,Chang, Yanyan,Zhang, Yun,Gao, Yuan,Zhi, Xiaoying,Lv, Jianliang,Wei, Yanquan,Du, Ping,Chang, Yanyan,Zhang, Yun,Gao, Yuan,Gao, Yuan. 2018

[10]The effects of ultrasound and arsenic trioxide on neurogliocytoma cells and secondary activation of macrophages. Xu, Yonggang,Liu, Xiaoqian,Wang, Zhi,Yue, Wu,Wang, Jie,Zhang, Yafang,Ma, Jing. 2009

[11]Salmonella Pullorum resistance in dwarf chickens selected for high macrophage phagocytosis. Ma, Hui,Lian, Z. X.,Han, H. B.,Yuan, Y. T.,Ning, Z. H.,Liu, W. B..

[12]Immunomodulatory activity on macrophage of a purified polysaccharide extracted from Laminaria japonica. Fang, Qing,Wang, Jing-Fei,Zha, Xue-Qiang,Cui, Shao-Hua,Luo, Jian-Ping,Cao, Lei.

[13]Macrophage immunomodulatory activity of polysaccharides isolated from Glycyrrhiza uralensis fish. Cheng, Anwei,Jin, Zhengyu,Xu, Xueming,Wan, Fachun,Wang, Jiaqi. 2008

[14]AMP-activated protein kinase attenuates oxLDL uptake in macrophages through PP2A/NF-kappa B/LOX-1 pathway. Chen, Bo,Li, Jin,Zhu, Haibo,Chen, Bo,Li, Jin,Zhu, Haibo,Chen, Bo,Li, Jin,Zhu, Haibo,Chen, Bo,Li, Jin,Zhu, Haibo,Li, Jin.

[15]ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages. Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Dai, Manman,Cao, Weisheng,Shi, Meiqing.

[16]PLC-gamma 1 is involved in the inflammatory response induced by influenza A virus H1N1 infection. Xu, Shuai,Yang, Jiayun,Zhu, Qiyun,Zhu, Liqian,Yuan, Chen,Ding, Xiuyan,Zhu, Liqian,Yuan, Chen,Ding, Xiuyan,Liang, Yuying.

[17]Analysis of the immune response in infections of the goldfish (Carassius auratus L.) with Mycobacterium marinum. Hodgkinson, Jordan W.,Ge, Jun-Qing,Grayfer, Leon,Stafford, James,Belosevic, Miodrag,Belosevic, Miodrag,Ge, Jun-Qing.

[18]EFFECTS OF TEN TRADITIONAL CHINESE HERBS ON IMMUNE RESPONSE AND DISEASE RESISTANCE OF SCIAENOPS OCELLATUS (ACTINOPTERYGII: PERCIFORMES: SCIAENIDAE). Pan, Tingshuang,Yan, Maocang,Chen, Shaobo,Pan, Tingshuang,Wang, Xuepeng. 2013

[19]Modulation of immune function by conjugated linoleic acid in chickens. He, Xi,Zhang, Shirui,Zhang, Haijun,Yang, Xiaojun,Xiao, Wenjun,Ren, Guopu. 2007

[20]CLASSIFICATION OF GRANULOCYTES IN THREE BIVALVE SPECIES USING NEUTRAL RED STAINING. Zhou, Kai. 2013

作者其他论文 更多>>

微信小程序