Effects of quorum quenching by AHL lactonase on AHLs, protease, motility and proteome patterns in Aeromonas veronii LP-11

文献类型: 外文期刊

第一作者: Gui, Meng

作者: Gui, Meng;Wu, Ruiyun;Liu, Lei;Li, Pinglan;Gui, Meng;Wang, Shun;Zhang, Li;Liu, Lei;Li, Pinglan;Wu, Ruiyun;Liu, Lei;Gui, Meng;Wang, Shun

作者机构:

关键词: Aeromonas veronii;Quorum sensing;Proteome;AHL lactonase;Specific spoilage organism

期刊名称:INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY ( 影响因子:5.277; 五年影响因子:5.388 )

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收录情况: SCI

摘要: Food spoilage by some bacteria is reported to be regulated by quorum sensing (QS). In this study, a quorum quenching approach was used to investigate the QS regulated phenotypes (growth, protease and motility) and proteins expression in of Aeromonas veronii. LP-11, which is a specific spoilage organism of sturgeon. AHL lactonase AiiA(A196) from Bacillus quenched the QS system, probably by enzymatically inactivating the AHLs produced by A. veronii LP-11. After AfiA(AI96) treatment, the protease and motility activities of A. veronii LP-11 were reduced, but cell growth was not affected. Proteome analysis revealed thirty-two proteins that were differentially expressed within cells treated with AiiA(AI96) at early stationary phase, and that are functionally involved in metabolite transport, amino acid metabolism, central metabolism, respiration, transcription and translation, suggesting that QS may globally coordinate the metabolic processes within A. veronii LP-11 cells. Some of these QS regulated proteins were identified to be potentially participated in nutrient acquirement from environment and spoilage behavior of the organism. Indeed, AiiA(AI96) treatment inhibited the spoilage progress of vacuum-packaged sturgeon stored at 4 degrees C. These results highlight that the QS is a major metabolism regulator within A. veronii LP-11 cells and participates in sturgeon spoilage.

分类号: TS201.3

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